| NaCl | 24.55 g |
| KCl | 0.75 g |
| NaHCO3 | 0.21 g |
| H3BO3 | 0.036 g |
| KBr | 0.1157 |
| NaF solution (2.94 g/L) | 1 ml |
| MgCl2·6 H2O | 4.067 g |
| MgSO4·7 H2O | 6.16 g |
| CaCl2·2 H2O | 1.47 g |
| SrCl2·6 H2O solution (17.33 g/L) | 1 ml |
| NaH2PO4·H2O solution (6.9 g/L) | 1 ml |
| Trace metals solution | 1 ml |
| Vitamin solution | 1 ml |
| Distilled H2O | to 1 L |
Filter sterilize. Do not autoclave.
Trace metal solution:
| Na2EDTA·2 H2O | 0.745 g |
| FeCl3·6 H2O solution (0.11 g/L) | 1 ml |
| MnCl2·4 H2O solution (3.96 g/L) | 1 ml |
| ZnSO4·7 H2O solution (1.15 g/L) | 1 ml |
| Na2MoO4·2 H2O solution (2.66 g/L) | 1 ml |
| CuSO4·5 H2O solution (2.23 g/L) | 1 ml |
| CoCl2·6 H2O solution (5.95 g/L) | 1 ml |
| Distilled water | to 1 L |
Vitamin solution:
| Thiamine·HCl | 100 mg |
| Biotin solution (0.5 g/L) | 1 ml |
| Cyanocobalamin solution (0.5 g/L) | 1 ml |
| Distilled water | to 1 L |
Chen, Y.-B., Zehr, J.P. & Mellon, M. 1996. Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium?Trichodesmium?sp. IMS 101 in defined media: evidence for a circadian rhythm. J. Phycol. 32: 916-923.
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