{"id":2280,"date":"2014-01-09T22:17:04","date_gmt":"2014-01-09T14:17:04","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=2280"},"modified":"2014-01-09T22:32:46","modified_gmt":"2014-01-09T14:32:46","slug":"ybc-ii-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/ybc-ii-medium.html","title":{"rendered":"YBC-II Medium"},"content":{"rendered":"

 <\/p>\n

Trichodesmium<\/i>\u00a0Medium\uff08\u675f\u6bdb\u85fb\u57f9\u517b\u57fa\uff09<\/h2>\n

 <\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
NaCl<\/td>\n24.55 g<\/td>\n<\/tr>\n
KCl<\/td>\n0.75 g<\/td>\n<\/tr>\n
NaHCO3<\/sub><\/td>\n0.21 g<\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/td>\n0.036 g<\/td>\n<\/tr>\n
KBr<\/td>\n0.1157<\/td>\n<\/tr>\n
NaF solution (2.94 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
MgCl2<\/sub>\u00b76 H2<\/sub>O<\/td>\n4.067 g<\/td>\n<\/tr>\n
MgSO4<\/sub>\u00b77 H2<\/sub>O<\/td>\n6.16 g<\/td>\n<\/tr>\n
CaCl2<\/sub>\u00b72 H2<\/sub>O<\/td>\n1.47 g<\/td>\n<\/tr>\n
SrCl2<\/sub>\u00b76 H2<\/sub>O solution (17.33 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
NaH2<\/sub>PO4<\/sub>\u00b7H2<\/sub>O solution (6.9 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Trace metals solution<\/td>\n1 ml<\/td>\n<\/tr>\n
Vitamin solution<\/td>\n1 ml<\/td>\n<\/tr>\n
Distilled H2<\/sub>O<\/td>\nto 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Filter sterilize. Do not autoclave.<\/p>\n

 <\/p>\n

Trace metal solution:<\/b><\/p>\n\n\n\n\n\n\n\n\n\n\n
Na2<\/sub>EDTA\u00b72 H2<\/sub>O<\/td>\n0.745 g<\/td>\n<\/tr>\n
FeCl3<\/sub>\u00b76 H2<\/sub>O solution (0.11 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
MnCl2<\/sub>\u00b74 H2<\/sub>O solution (3.96 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
ZnSO4<\/sub>\u00b77 H2<\/sub>O solution (1.15 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>\u00b72 H2<\/sub>O solution (2.66 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
CuSO4<\/sub>\u00b75 H2<\/sub>O solution (2.23 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
CoCl2<\/sub>\u00b76 H2<\/sub>O solution (5.95 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Distilled water<\/td>\nto 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Vitamin solution:<\/b><\/p>\n\n\n\n\n\n\n
Thiamine\u00b7HCl<\/td>\n100 mg<\/td>\n<\/tr>\n
Biotin solution (0.5 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Cyanocobalamin solution (0.5 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Distilled water<\/td>\nto 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

Chen, Y.-B., Zehr, J.P. & Mellon, M. 1996. Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium\u00a0Trichodesmium<\/i>\u00a0sp. IMS 101 in defined media: evidence for a circadian rhythm. J. Phycol. 32: 916-923.<\/p>\n

 <\/p>\n

ybc-II medium<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

  Trichodesmium\u00a0Medium\uff08\u675f\u6bdb\u85fb\u57f9\u517b\u57fa\uff09   NaCl 24.55 g KCl 0.75 g NaHCO3 0.21 g H3BO3 0.036 g KBr 0.1157 NaF solution (2.94 g\/L) 1 ml MgCl2\u00b76 H2O 4.067 g MgSO4\u00b77 H2O 6.16 g CaCl2\u00b72 H2O 1.47 g SrCl2\u00b76 […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[307,306,371,308],"class_list":["post-2280","post","type-post","status-publish","format-standard","hentry","category-medium","tag-trichodesmium","tag-ybc-ii","tag-medium","tag-308"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2280"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=2280"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2280\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=2280"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=2280"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=2280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}