{"id":2440,"date":"2014-03-15T14:53:09","date_gmt":"2014-03-15T06:53:09","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=2440"},"modified":"2014-03-15T14:54:42","modified_gmt":"2014-03-15T06:54:42","slug":"hgz-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/hgz-medium.html","title":{"rendered":"HGZ\u57f9\u517b\u57fa"},"content":{"rendered":"

\u7ecf\u4fee\u6539\u7684HGZ-145\u57f9\u517b\u57fa\u914d\u65b9\u7528\u4e8e\u96e8\u751f\u7ea2\u7403\u85fb\u57f9\u517b\u3002<\/p>\n

The\u00a0components\u00a0of\u00a0the\u00a0amended\u00a0HGZ\u00a0-145\u00a0culture\u00a0medium<\/p>\n\n\n\n\n\n\n\n\n\n\n
\u5e38\u91cf\u5143\u7d20<\/td>\n\u542b\u91cf\uff08g\/L\uff09<\/td>\n\u5fae\u91cf\u5143\u7d20<\/td>\n\u542b\u91cf\uff08g\/L\uff09<\/td>\n<\/tr>\n
NaNO3<\/td>\n0.2\uff08<\/span>0.4*<\/span>\uff09<\/span><\/td>\nHBO3<\/td>\n0.00286<\/td>\n<\/tr>\n
KNO3<\/td>\n0.051<\/td>\nMnCl2\u00b74H2O<\/td>\n0.00181<\/td>\n<\/tr>\n
K2HPO4<\/td>\n0.049<\/td>\nZnSO4\u00b77H2O<\/td>\n0.000222<\/td>\n<\/tr>\n
MgSO4\u00b77H2O<\/td>\n0.075<\/td>\nNa2Mo4\u00b72H2O<\/td>\n0.000391<\/td>\n<\/tr>\n
Na2CO3<\/td>\n0.02<\/td>\nCuSO4\u00b75H2O<\/td>\n0.000079<\/td>\n<\/tr>\n
Ca(NO3)2\u00b74H2O<\/td>\n0.059<\/td>\nFe-EDTA<\/td>\n0.000932<\/td>\n<\/tr>\n
NH4Cl(NH4Cl-\u00a0EDTA)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a00.0391**<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

*\uff1a\u5171\u57f9\u6a21\u62df\u6761\u4ef6\u4e0b\uff0cNaNO3\u7684\u5bc6\u5ea6\u3002<\/p>\n

**:\u00a0NH4Cl(NH4Cl-\u00a0EDTA)\u4e0eFe-EDTA\u914d\u7f6e\u7684\u65b9\u6cd5\u53c2\u7167\u6587\u732e\u53f6\u5c45\u65b0\u7b49\uff081999\uff09\u3002<\/p>\n

\u6839\u636e\u4e0a\u8ff0\u57f9\u517b\u57fa\u914d\u65b9\uff0c\u914d\u597d\u6bcf\u4e00\u79cd\u5e38\u91cf\u8425\u517b\u76d0\u7c7b1000\u500d\u7684\u6d53\u7f29\u50a8\u5907\u6db2\u3002\u540c\u65f6\u914d\u5236\u5fae\u91cf\u8425\u517b\u6df7\u5408\u76d0\u7c7b1000\u500d\u7684\u6d53\u7f29\u50a8\u5907\u6db2TMS<\/span>\uff08<\/span>Trace\u00a0Metal\u00a0Solution<\/span>\uff09\u3002\u5728<\/span>900ml\u84b8\u7559\u6c34\u4e2d\u4f9d\u6b21\u52a0\u5165\u5404\u50a8\u5907\u6db21ml\uff0c\u6dfb\u52a0\u987a\u5e8f\u4e3a\uff1aNaNO3(10ml)\u2192<\/span>KNO3\u2192K2HPO4\u2192MgSO<\/span>4\u00b77H2O\u2192<\/span>Na2CO3\u2192Ca(NO3)2\u00b74H2O\u2192NH4Cl(NH4Cl-\u00a0EDTA)\u2192Fe-EDTA\u00a0\u00a0\u2192TMS<\/span>\u3002\u6bcf\u52a0\u5165\u4e00\u79cd\u8425\u517b\u76d0\u5fc5\u987b\u5145\u5206\u6447\u52a8\u5bb9\u91cf<\/span>\u74f6\uff0c\u4f7f\u6df7\u5408\u5747\u5300\u540e\u518d\u52a0\u5165\u4e0b\u4e00\u79cd\u3002<\/p>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n
Trace Metals Solution<\/b>\u00a0\u00a0<\/span><\/td>\n<\/tr>\n
Directions\u00a0<\/b><\/td>\n<\/tr>\n
For 1 L Total
\n1. Begin stirring and heating approximately 950 mL of dH2<\/sub>O.
\n2. When the stirring water approaches the boiling point, add the following components in the order listed.
\n3. Bring the volume to 1 L with dH2<\/sub>O.
\n4. Store at refrigerator temperature.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
<\/td>\n\n\n\n\n\n\n\n\n\n\n\n
#<\/td>\nComponent<\/td>\nAmount<\/td>\nStock Solution
\nConcentration<\/td>\n		Final Concentration<\/td>\n<\/tr>\n
1<\/td>\nZnSO4<\/sub>\u00b7<\/b>7H2<\/sub>O (Sigma Z 0251)<\/td>\n23 mg\/L<\/td>\n<\/td>\n0.08 \u00b5M<\/td>\n<\/tr>\n
2<\/td>\nMnSO4<\/sub>\u00b7<\/b>H2<\/sub>O (Sigma M8179)<\/td>\n152 mg\/L<\/td>\n<\/td>\n0.9 \u00b5M<\/td>\n<\/tr>\n
3<\/td>\nNa2<\/sub>MoO4<\/sub>\u00b7<\/b>2H2<\/sub>O (J.T. Baker 3764)<\/td>\n7.3 mg\/L<\/td>\n<\/td>\n0.03 \u00b5M<\/td>\n<\/tr>\n
4<\/td>\nCoSO4<\/sub>\u00b7<\/b>7H2<\/sub>O (Baker 1696)<\/td>\n14 mg\/L<\/td>\n<\/td>\n0.05 \u00b5M<\/td>\n<\/tr>\n
5<\/td>\nCuCl2<\/sub>\u00b7<\/b>2H2<\/sub>O (Fisher C-455)<\/td>\n6.8 mg\/L<\/td>\n<\/td>\n0.04 \u00b5M<\/td>\n<\/tr>\n
6<\/td>\nFe(NH4<\/sub>)2<\/sub>(SO4<\/sub>)2<\/sub>\u00b7<\/b>6H2<\/sub>O (Sigma F-1513)<\/td>\n4.6 g\/L<\/td>\n<\/td>\n11.7 \u00b5M<\/td>\n<\/tr>\n
7<\/td>\nNa2<\/sub>EDTA\u00b72H2<\/sub>O (Sigma ED255)<\/td>\n4.4 g\/L<\/td>\n<\/td>\n11.7 \u00b5M<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

\u7ecf\u4fee\u6539\u7684HGZ-145\u57f9\u517b\u57fa\u914d\u65b9\u7528\u4e8e\u96e8\u751f\u7ea2\u7403\u85fb\u57f9\u517b\u3002<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[319],"class_list":["post-2440","post","type-post","status-publish","format-standard","hentry","category-medium","tag-hgz"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2440"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=2440"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2440\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=2440"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=2440"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=2440"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}