{"id":2942,"date":"2015-03-17T21:52:44","date_gmt":"2015-03-17T13:52:44","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=2942"},"modified":"2015-03-17T21:52:44","modified_gmt":"2015-03-17T13:52:44","slug":"pro99-meida","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/pro99-meida.html","title":{"rendered":"Pro99\u57f9\u517b\u57fa\uff08Prochlorococcus \u539f\u7eff\u7403\u85fb\u57f9\u517b\u57fa\uff09"},"content":{"rendered":"<table border=\"1\">\n<tbody>\n<tr>\n<td>NaH<sub>2<\/sub>PO<sub>4<\/sub>\u00b7H<sub>2<\/sub>O solution (6.9 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>NH<sub>4<\/sub>Cl solution (42.8 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>Trace element solution<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>Oligotrophic seawater<\/td>\n<td>to 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Acid wash and dH<sub>2<\/sub>O rinse all glassware. Use oligotrophic (Sargasso Sea) seawater. Autoclave and cool before adding filter-sterilized nutrient stock solutions.<\/p>\n<p><b>Trace element solution:<\/b><\/p>\n<table border=\"1\">\n<tbody>\n<tr>\n<td>Na<sub>2<\/sub>EDTA\u00b72 H<sub>2<\/sub>O<\/td>\n<td>0.436 g<\/td>\n<\/tr>\n<tr>\n<td>FeCl<sub>3<\/sub>\u00b76 H<sub>2<\/sub>O<\/td>\n<td>0.316 g<\/td>\n<\/tr>\n<tr>\n<td>ZnSO<sub>4<\/sub>\u00b77 H<sub>2<\/sub>O solution (2.3 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>CoCl<sub>2<\/sub>\u00b76 H<sub>2<\/sub>O solution (1.19 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>MnCl<sub>2<\/sub>\u00b74 H<sub>2<\/sub>O solution (17.8 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>Na<sub>2<\/sub>MoO<sub>4<\/sub>\u00b72 H<sub>2<\/sub>O solution (0.73 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>Na<sub>2<\/sub>SeO<sub>3<\/sub> solution (1.29 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>NiSO<sub>4<\/sub>\u00b76 H<sub>2<\/sub>O solution (2.63 g\/L)<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>Distilled water<\/td>\n<td>to 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>NaH2PO4\u00b7H2O solution (6.9 g\/L) 1 ml NH4Cl solution (42.8 g\/L) 1 ml Trace element solution 1 ml Oligotrophic seawater to 1 L Acid wash and dH2O rinse all glassware. Use oligotrophic (Sargasso Sea) seaw [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[371],"class_list":["post-2942","post","type-post","status-publish","format-standard","hentry","category-medium","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2942"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=2942"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2942\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=2942"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=2942"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=2942"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}