{"id":2964,"date":"2015-04-03T07:53:29","date_gmt":"2015-04-02T23:53:29","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=2964"},"modified":"2015-04-03T07:57:58","modified_gmt":"2015-04-02T23:57:58","slug":"c-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/c-medium.html","title":{"rendered":"C\u57f9\u517b\u57fa"},"content":{"rendered":"

Media for freshwater, terrestrial, hot spring and salt water algae<\/strong><\/p>\n

C<\/h1>\n

Ca(NO3)2 \u00b7 4H2O 15 mg<\/p>\n

KNO3 10 mg<\/p>\n

\u03b2-Na2glycerophosphate \u00b7 5H2O 5 mg<\/p>\n

MgSO4 \u00b7 7H2O 4 mg<\/p>\n

Vitamin B12 0.01 \u00b5g<\/p>\n

Biotin 0.01 \u00b5g<\/p>\n

Thiamine HCl 1 \u00b5g<\/p>\n

PIV metals 0.3 mL<\/p>\n

Tris (hydroxymethyl) aminomethane 50 mg<\/p>\n

Distilled water 99.7 mL<\/p>\n

pH 7.5<\/p>\n

Add 1.5 g agar to 100 mL of medium to give a solid medium.<\/p>\n

Reference <\/strong><\/h2>\n

Ichimura, T. 1971 Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In Proceedings of the Seventh International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 208-214.<\/p>\n

 <\/p>\n

PIV metals <\/strong><\/h1>\n

Na2EDTA \u00b7 2H2O 100 mg<\/p>\n

FeCl3 \u00b7 6H2O 19.6 mg<\/p>\n

MnCl2 \u00b7 4H2O 3.6 mg<\/p>\n

ZnCl2 1) 1.04 mg<\/p>\n

CoCl2 \u00b7 6H2O 0.4 mg<\/p>\n

Na2MoO4 \u00b7 2H2O 0.25 mg<\/p>\n

Distilled water 100 mL<\/p>\n

1) In the NIES-Collection, 1.04 mg ZnCl2 is replaced by 2.2mg ZnSO4 \u00b7 7H2O.<\/p>\n

Reference <\/strong><\/h2>\n

Provasoli, L., Pintner, I. J. 1959 Artificial media for fresh-water algae: problems and suggestions. In The Ecology of Algae. Spec. Pub. No. 2,, Eds. by Tryon, C. A., Jr. & Hartmann, R. T., Pymatuning Laboratory of Field Biology, University of Pittsburgh, Pittsburgh, p. 84-96.<\/p>\n","protected":false},"excerpt":{"rendered":"

It’s a media for freshwater, terrestrial, hot spring and salt water algae<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[371],"class_list":["post-2964","post","type-post","status-publish","format-standard","hentry","category-medium","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2964"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=2964"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2964\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=2964"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=2964"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=2964"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}