{"id":2966,"date":"2015-04-03T07:56:56","date_gmt":"2015-04-02T23:56:56","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=2966"},"modified":"2015-04-03T07:57:10","modified_gmt":"2015-04-02T23:57:10","slug":"esm-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/esm-medium.html","title":{"rendered":"ESM\u57f9\u517b\u57fa"},"content":{"rendered":"<p>Media for marine and brackish water microalgae<\/p>\n<h1>ESM<\/h1>\n<p>NaNO3 12 mg<\/p>\n<p>K2HPO4 0.5 mg<\/p>\n<p>Vitamin B12 0.1 \u00b5g<\/p>\n<p>Biotin 0.1 \u00b5g<\/p>\n<p>Thiamine HCl 10 \u00b5g<\/p>\n<p>Fe &#8211; EDTA 25.9 \u00b5g<\/p>\n<p>Mn &#8211; EDTA 33.2 \u00b5g<\/p>\n<p>Tris (hydroxymethyl) aminomethane 100 mg<\/p>\n<p>Soil extract 1) 2.5 mL<\/p>\n<p>Seawater 97.5 mL<\/p>\n<p>pH 8.0<\/p>\n<p>Add 1.5 g agar to 100 mL of medium to give a solid medium.<\/p>\n<p>1) The amount of soil extract depends on the quality of the soil. In the NIES-Collection, soil extract was reduced from 5 mL to 2.5 mL after 2002.<\/p>\n<h2>Reference<\/h2>\n<p>Okaichi, T., Nishio, S., Imatomi, Y. 1982 Collection and mass culture [Shiry\u00f4 no saisyu to baiy\u00f4]. In Toxic phytoplankton &#8211; Occurrence, mode of action, and toxins [Y\u00fbdoku Purankuton -Hassei, Say\u00f4kik\u00f4, Dokuseibun], Ed. by Jpn. Fish. Soc., K\u00f4seisya-K\u00f4seikaku, Tokyo, p. 22-34 (in Japanese without English title).<\/p>\n<p>&nbsp;<\/p>\n<h1>Soil extract<\/h1>\n<p>To 1000 mL distilled water add 200 mL of soil (soil from undisturbed deciduous woodland is best) and heat by autoclaving for 1 h at 105\u00b0C. When cool, heat by autoclaving for 1 h at 105\u00b0C again. Pass the supernatant through a GF\/C filter and Celite, and then pass the filtrate through a GF\/F filter. Adjust to 1000 mL by adding distilled water. Dispense 10 mL of the final filtrate into each test tube and sterilize by autoclaving for 20 min at 121\u00b0C. Keep in a cool place.<\/p>\n<h2>Reference<\/h2>\n<p>Provasoli, L., McLaughlin, J. J. A., Droop, M. R. 1957 The development of artificial media for marine algae. Arch. Mikrobiol., 25, 392-428.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Esm medium is a media for marine and brackish water microalgae<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[371],"class_list":["post-2966","post","type-post","status-publish","format-standard","hentry","category-medium","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2966"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=2966"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2966\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=2966"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=2966"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=2966"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}