{"id":3312,"date":"2015-11-06T16:04:39","date_gmt":"2015-11-06T08:04:39","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=3312"},"modified":"2015-11-06T16:05:04","modified_gmt":"2015-11-06T08:05:04","slug":"f2-derivatives","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/f2-derivatives.html","title":{"rendered":"F\/2\u884d\u751f\u57f9\u517b\u57fa\u7cfb\u5217"},"content":{"rendered":"

f\/2\u57f9\u517b\u57fa\u662f\u76ee\u524d\u5fae\u85fb\u4fdd\u79cd\u57f9\u517b\u8fc7\u7a0b\u4e2d\u7528\u5230\u6700\u5e7f\u6cdb\u7684\u914d\u65b9\u4e4b\u4e00\uff0c\u9488\u5bf9\u4e0d\u540c\u7684\u4e00\u4e9b\u85fb\u4ee5f\/2\u57f9\u517b\u57fa\u4e3a\u57fa\u7840\u8fd8\u80fd\u884d\u751f\u51fa\u4e00\u4e9b \u7279\u6b8a\u7528\u9014\u7684\u914d\u65b9\u3002<\/span><\/p>\n

Black Sea Medium<\/strong>: For brackish water organisms (16 psu, half-strength nutrients). Combine 500 mL f\/2 medium and 500 mL dH2O. Autoclave.<\/span><\/p>\n

f\/2 agar<\/strong>: Prepare 1 liter of f\/2 medium and dissolve 9g Bacto-agar (heat and mix). For test tubes, dispense dissolved agar medium into tubes, autoclave, and then cool with tubes slanted at an angle. For Petri plates, autoclave in a flask, cool almost to the gelling point, and then aseptically dispense into sterile Petri plates. Note: Agar can be added to other media (e.g., f\/50 agar), and agar concentration can be varied to produce softer or firmer substrates.<\/span><\/p>\n

f\/2-Si<\/strong>: Prepare as for f\/2 medium but omit Na2SiO 3 \u00b7 9H2O. This is preferred over f\/2 medium for organisms with no silica requirement because less precipitation forms.<\/span><\/p>\n

f\/2 + Se<\/strong>: Extra silicon and selenium are beneficial to several diatom strains. Prepare 1 L of f\/2 medium but use 2 mL of silicate stock, then add 1.0 mL of selenium stock solution (1.29 mg H2SeO 3 \/L distilled H2O). Autoclave.<\/span><\/p>\n

f\/2 (11 psu)<\/strong>: For brackish water organisms. Mix 650 mL distilled H2O and 350 mL filtered seawater. Add f\/2 medium nutrients and autoclave.<\/span><\/p>\n

f\/2-Si (24 psu)<\/strong>: Mix 750 mL distilled H2O and 250 mL filtered seawater. Prepare as for f\/2 medium but omit Na2SiO 3 \u00b7 9H2O.<\/span><\/p>\n

f\/4<\/strong>: Add 500 mL f\/2 medium to 500 mL filtered seawater, then autoclave.<\/span><\/p>\n

f\/4-Si<\/strong>: Autoclave 1 L of filtered seawater. When cool, aseptically add f\/2-Si nutrients at half concentration (i.e., 0.5 mL).<\/span><\/p>\n

f\/20-Si<\/strong>: Autoclave 1 L of filtered seawater. When cool, aseptically add f\/2-Si nutrients at one tenth concentration (i.e., 100 \u03bcL).<\/span><\/p>\n

f\/50-Si<\/strong>: This is more than a 1\/25 dilution of f\/2-Si medium. We autoclave 1 L of seawater in a Teflon-lined bottle. Wait for the autoclaved seawater to cool to room temperature (important). Aseptically add 40 \u03bcL of sterile f\/2 nutrients (20 \u03bcL of vitamins).<\/span><\/p>\n

f\/50-Si + CCMP1320 as food<\/strong>: Prepare f\/50 and aseptically add 50 \u03bcL of healthy, moderately dense culture of CCMP1320.<\/span><\/p>\n

f\/2m<\/strong>: To 1L f\/2 medium add 1 g methylamine \u00b7 HCl, mix until dissolved and autoclave. This medium is used to test for contamination by methylaminotrophic bacteria.<\/span><\/p>\n

f\/2p<\/strong>: To 1 L f\/2 medium, add 1 g Bacto-peptone, mix until dissolves and autoclave. This medium is used to test for contamination by non- methylaminotrophic bacteria and fungi.<\/span><\/p>\n

f\/2pm<\/strong>: To 1L f\/2 medium add 1 g Bacto-peptone and 1 g methylamine \u00b7 HCl, mix until dissolved and autoclave. This general medium is used to test for contamination by bacteria and fungi.<\/span><\/p>\n

f\/2 + NPM<\/strong>: Add f\/2 nutrients to 900 mL of seawater and autoclave. After cooling, aseptically add 100 mL of the following organic stock solution. Dispense aseptically into test tubes.<\/span><\/p>\n

\n

 <\/p>\n

Organics\u00a0Stock\u00a0Solution<\/b>\u00a0<\/b><\/span><\/p>\n

(modified\u00a0from\u00a0Guillard\u00a01960)<\/span><\/p>\n

To\u00a0900\u00a0mL\u00a0dH2O\u00a0add:<\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Quantity<\/b><\/span><\/td>\nCompound<\/b><\/span><\/td>\n<\/tr>\n
1\u00a0g<\/span><\/td>\nsodium\u00a0acetate<\/span><\/td>\n<\/tr>\n
6\u00a0g<\/span><\/td>\nglucose<\/span><\/td>\n<\/tr>\n
3\u00a0g<\/span><\/td>\n(di-)\u00a0sodium\u00a0succinate\u00a0\u00b7\u00a06H2O<\/span><\/td>\n<\/tr>\n
4\u00a0g<\/span><\/td>\nneopeptone<\/span><\/td>\n<\/tr>\n
1\u00a0g<\/span><\/td>\nBacto-tryptone<\/span><\/td>\n<\/tr>\n
100\u00a0mg<\/span><\/td>\nyeast\u00a0extract<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Bring\u00a0up\u00a0to\u00a01\u00a0L\u00a0with\u00a0dH2O.\u00a0Dispense\u00a0in\u00a0small\u00a0aliquots\u00a0and\u00a0autoclave.<\/span><\/p>\n

 <\/p>\n

References<\/strong><\/span>
\n Guillard, R.R.L. 1960. A mutant of Chlamydomonas moewusii lacking contractile vacuoles. J. Protozool.<\/strong> 7: 262-268.<\/span><\/p>\n

Guillard, R.R.L. 1975. Culture of phytoplankton for feeding marine invertebrates. pp 26-60. In Smith, W.L. and Chanle,y M.H. (eds.) Culture of Marine Invertebrate Animals.<\/span> Plenum Press, New York, USA.<\/span><\/p>\n

Guillard, R.R.L. and Ryther, J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol<\/strong>. 8: 229-239.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

f\/2\u57f9\u517b\u57fa\u662f\u76ee\u524d\u5fae\u85fb\u4fdd\u79cd\u57f9\u517b\u8fc7\u7a0b\u4e2d\u7528\u5230\u6700\u5e7f\u6cdb\u7684\u914d\u65b9\u4e4b\u4e00\uff0c\u9488\u5bf9\u4e0d\u540c\u7684\u4e00\u4e9b\u85fb\u4ee5f\/2\u57f9\u517b\u57fa\u4e3a\u57fa\u7840\u8fd8\u80fd\u884d\u751f\u51fa\u4e00\u4e9b \u7279\u6b8a\u7528\u9014\u7684\u914d\u65b9\u3002<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[],"class_list":["post-3312","post","type-post","status-publish","format-standard","hentry","category-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/3312"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=3312"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/3312\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=3312"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=3312"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=3312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}