\u76ee\u524d\u7814\u7a76\u663e\u793a\uff0c\u5e03\u6717\u8461\u8404\u85fb\u7684\u542b\u6cb9\u91cf\u975e\u5e38\u9ad8\uff0c\u80fd\u591f\u75286\u5929\u5de6\u53f3\u7684\u65f6\u95f4\u589e\u6b961\u500d\uff0c\u800c\u8fd1\u671f\u65e5\u672c\u53d1\u73b0\u7684Aurantiochytrium\u4ec5\u75284\u4e2a\u5c0f\u65f6\u5c31\u80fd\u589e\u6b961\u500d\u3002<\/p>\n
\u65e5\u672c\u7684\u5f7c\u8c37\u6559\u6388\u7b49\u6b63\u5728\u7814\u7a76\u7684\u662f\u7ed3\u5408\u5229\u7528\u5e03\u6717\u8461\u8404\u85fb\u548cAurantiochytrium\u4e24\u8005\u7684\u201c\u6df7\u5408\u7e41\u6b96\u201d\u3002\u63d0\u53d6\u6cb9\u5206\u4e4b\u540e\u7684\u5e03\u6717\u8461\u8404\u85fb\u201c\u6b8b\u6e23\u201d\uff0c\u4ee5\u53ca\u5e03\u6717\u8461\u8404\u85fb\u7e41\u6b96\u8fc7\u7a0b\u4e2d\u5206\u6ccc\u51fa\u7684\u7cd6\u5206\u4f1a\u6210\u4e3aAurantiochytrium\u7684\u517b\u5206\u3002\u4e5f\u5c31\u662f\u5229\u7528\u5e03\u6717\u8461\u8404\u85fb\u7684\u7e41\u6b96\u8fdb\u884cAurantiochytrium\u7684\u7e41\u6b96\u3002<\/p>\n
\u867d\u7136\u5b58\u5728\u5982\u4f55\u63d0\u4f9b\u7e41\u6b96\u6240\u9700\u201c\u8425\u517b\u201d\u7684\u95ee\u9898\uff0c\u4f46\u591c\u95f4\u4e5f\u53ef\u7e41\u6b96\u7684\u85fb\u7c7b\u7684\u5b58\u5728\uff0c\u4e3a\u5168\u9762\u5927\u91cf\u751f\u4ea7\u71c3\u6599\u63d0\u4f9b\u4e86\u66f4\u591a\u7684\u53ef\u80fd\u6027\u3002\u201d\u7531\u4e8e\u8fd9\u79cd\u85fb\u7c7b\u7e41\u6b96\u901f\u5ea6\u975e\u5e38\u5feb\uff0c\u56e0\u6b64\u5982\u679c\u80fd\u591f\u4ee5\u4f4e\u6210\u672c\u63d0\u4f9b\u517b\u5206\uff0c\u4fbf\u53ef\u5b9e\u73b0\u71c3\u6599\u7684\u4f4e\u6210\u672c\u5316\u53ca\u5927\u91cf\u751f\u4ea7\u7cfb\u7edf\u3002\u751a\u81f3\u8003\u8651\u5230\u5229\u7528\u4e0b\u6c34\u6c61\u6ce5\u548c\u98df\u54c1\u5de5\u5382\u5e9f\u6c34\u7b49\u542b\u6709\u6709\u673a\u7269\u7684\u5e9f\u5f03\u7269\uff0c\u5927\u90e8\u5206\u90fd\u80fd\u6210\u4e3aAurantiochytrium\u7684\u8425\u517b\u3002<\/p>\n
<\/p>\n
\u9644\u6ce8\uff1a<\/strong><\/p>\n http:\/\/etds.lib.ncku.edu.tw\/etdservice\/view_metadata?etdun=U0026-1808201219055900<\/p>\n \u6765\u81ea\u5317\u53f0\u6e7e\u4e4b\u6d77\u6d0b\u5f02\u8425\u6027\u5fae\u85fb\u5206\u79bb\u682a:Aurantiochytrium sp. strain BL10\u4e4b\u751f\u7269\u7279\u6027\u7814\u7a76\uff08\u4f5c\u8005\uff1a\u82cf\u6631\u94ed\uff09<\/strong><\/p>\n BL10\u662f\u4e00\u4e2a\u6765\u81ea\u53f0\u6e7e\u5317\u90e8\u6cb3\u53e3\u7684\u5f02\u8425\u6027\u5fae\u85fb\u5206\u79bb\u682a\uff0c\u5177\u6709\u8bb8\u591a\u5f02\u65bc\u5176\u4ed6\u5fae\u7ec6\u85fb\u7c7b\u7684\u7279\u6027\uff1a1. \u5e7f\u76d0\u6027 (euryhaline)\uff0c\u57282 – 35 ppt\u7684\u76d0\u5ea6\u8303\u56f4\u5185\u6709\u6700\u4f73\u7684\u751f\u957f\u901f\u7387\u30022. \u55dc\u7cd6\u6027 (saccharophilic)\uff0c\u5176\u751f\u957f\u4e0d\u53d7\u9ad8\u6d53\u5ea6\u8461\u8404\u7cd6 (140 gL-1) \u7684\u6291\u5236\u30023. \u6cb9\u8102\u6027 (oleaginous)\uff0c\u542b\u6cb9\u91cf\u53ef\u8fbe\u4e7e\u91cd\u768480%\u30024. \u5bb9\u6613\u57f9\u517b\uff0c\u4ee50.01gL-1\u63a5\u79cd\uff0c\u57f9\u517b72\u5c0f\u65f6\u5f8c\uff0c\u53ef\u8fbe120 gL-1\u7684\u751f\u7269\u91cf\u30025. \u6709\u522b\u65bc\u6807\u51c6\u8def\u5f84\u7684\u957f\u94fe\u4e0d\u9971\u548c\u8102\u80aa\u9178\u751f\u5408\u6210\u8def\u5f84\u30026. \u7279\u6b8a\u7684\u751f\u6d3b\u53f2\uff0c\u9664\u4e86\u8425\u517b\u7ec6\u80de (vegetative cell) \u5916\uff0c\u5c1a\u80fd\u4ea7\u751f\u5177\u6709\u6e38\u52a8\u80fd\u529b\u7684\u6e38\u8d70\u5b62\u5b50 (zoospore) \u4ee5\u53ca\u722c\u884c\u80fd\u529b\u7684\u963f\u7c73\u5df4\u7ec6\u80de (amoeboid cell)\u3002\u56e0\u6b64\uff0c\u6216\u8bb8\u80fd\u5c06BL10\u5efa\u7acb\u4e3a\u65b0\u578b\u7684\u6a21\u5f0f\u751f\u7269\uff0c\u7528\u4ee5\u7814\u7a76\u5fae\u85fb\u7684\u6e17\u900f\u538b\u8c03\u8282\u3001\u6cb9\u8102\u4ea7\u751f\u53ca\u5806\u79ef\u3001\u957f\u94fe\u4e0d\u9971\u548c\u8102\u80aa\u9178\u7684\u751f\u5408\u6210\u8def\u5f84\u3001\u4ee5\u53ca\u7ec6\u80de\u8fc1\u5f99\u7b49\u751f\u547d\u73b0\u8c61\u3002\u7136\u800c\u6b32\u4ee5\u5176\u4e3a\u6a21\u5f0f\u751f\u7269\uff0c\u5fc5\u5148\u4e86\u89e3\u5176\u5c5e\u65bc\u54ea\u79cd\u85fb\u3001\u80fd\u5426\u53d6\u5f97\u5168\u57fa\u56e0\u4f53\u7684\u8d44\u6599\uff0c\u4ee5\u53ca\u662f\u5426\u5bb9\u6613\u8fdb\u884c\u57fa\u56e0\u64cd\u4f5c\u3002 \u76ee\u524d\u7814\u7a76\u663e\u793a\uff0c\u5e03\u6717\u8461\u8404\u85fb\u7684\u542b\u6cb9\u91cf\u975e\u5e38\u9ad8\uff0c\u80fd\u591f\u75286\u5929\u5de6\u53f3\u7684\u65f6\u95f4\u589e\u6b961\u500d\uff0c\u800c\u8fd1\u671f\u65e5\u672c\u53d1\u73b0\u7684Aurantiochytrium\u4ec5\u75284\u4e2a\u5c0f\u65f6\u5c31\u80fd\u589e\u6b961\u500d\u3002 \u65e5\u672c\u7684\u5f7c\u8c37\u6559\u6388\u7b49\u6b63\u5728\u7814\u7a76\u7684\u662f\u7ed3\u5408\u5229\u7528\u5e03\u6717\u8461\u8404\u85fb\u548cAurantiochytrium\u4e24\u8005\u7684\u201c\u6df7\u5408\u7e41\u6b96\u201d\u3002\u63d0\u53d6\u6cb9\u5206\u4e4b\u540e\u7684\u5e03\u6717\u8461\u8404\u85fb\u201c\u6b8b\u6e23\u201d\uff0c\u4ee5\u53ca\u5e03\u6717\u8461\u8404\u85fb\u7e41\u6b96\u8fc7\u7a0b\u4e2d\u5206\u6ccc\u51fa\u7684\u7cd6\u5206\u4f1a\u6210\u4e3aAurantiochytrium\u7684\u517b\u5206\u3002\u4e5f\u5c31\u662f\u5229\u7528\u5e03\u6717\u8461\u8404\u85fb\u7684\u7e41\u6b96\u8fdb\u884cAura […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[12,4],"tags":[20],"class_list":["post-373","post","type-post","status-publish","format-standard","hentry","category-technology","category-info","tag-20"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/373"}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=373"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/373\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=373"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=373"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=373"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n\u56e0\u6b64\uff0c\u672c\u7814\u7a76\u7684\u76ee\u7684\uff0c\u5728\u65bc\u786e\u8ba4BL10\u7684\u5206\u7c7b\u5730\u4f4d\u3001\u8bc4\u4f30\u4e0e\u5206\u6790\u57fa\u56e0\u4f53\u5927\u5c0f\u53ca\u67d3\u8272\u4f53\u6838\u578b (karyotype)\uff0c\u4ee5\u53ca\u7814\u62df\u5168\u57fa\u56e0\u4f53\u5b9a\u5e8f\u53ca\u57fa\u56e0\u64cd\u4f5c\u7684\u7b56\u7565\u3002\u9996\u5148\u4ee518S rDNA\u7684\u5e8f\u5217\u4e0e\u5df2\u77e5Aurantiochytrium\u4ee5\u53ca\u5176\u4ed6\u7834\u56ca\u58f6\u83cc\u54c1\u7cfb\u8fdb\u884c\u4eb2\u7f18\u5173\u7cfb\u5206\u6790\u65f6\uff0c\u53d1\u73b0BL10\u4f1a\u4e0e\u5df2\u77e5\u7684Aurantiochytrium\u54c1\u7cfb\u5f62\u6210\u5355\u7cfb\u7c7b\u7fa4 (monophyletic group) \u540c\u65f6\u548cA. limacinum\u7684type species SR21\uff0c\u800c\u975eA. mangrovei\u7684type species RCC893\u6709\u8f83\u8fd1\u4f3c\u7684\u4eb2\u7f18\u5173\u7cfb\u3002BL10\u5bb9\u6613\u89c2\u5bdf\u5230\u963f\u7c73\u5df4\u7ec6\u80de\uff0c\u4ee5\u53ca\u963f\u7c73\u5df4\u7ec6\u80de\u5728\u505c\u6b62\u79fb\u52a8\u5f8c\u4e0d\u4f1a\u7acb\u5373\u5f62\u6210\u6e38\u8d70\u5b62\u5b50\u7684\u5f62\u6001\u7279\u5fb5\u4e5f\u548cA. limacinum\u8fd1\u4f3c\uff0c\u7136\u800cBL10\u4e0d\u4f1a\u5f62\u6210\u5b62\u5b50\u56ca\uff0c\u4ee5\u53ca\u5355\u4e00\u8425\u517b\u7ec6\u80de\u5f62\u6210\u5b62\u5b50\u56ca\u7684\u7279\u5fb5\u4e0eA. limacinum\u8fe5\u5f02\u3002\u56e0\u6b64\u672c\u7814\u7a76\u4ecd\u6682\u65f6\u5c06BL10\u5b9a\u540d\u4e3aAurantiochytrium sp.\u3002
\n\u6b64\u5916\uff0c\u6211\u4eec\u5229\u7528\u6dfb\u52a00.2% Triton X-100\u7684\u65b9\u6cd5\uff0c\u8bc1\u660e\u963f\u7c73\u5df4\u7ec6\u80de\u4e3aBL10\u7684\u4e09\u79cd\u7ec6\u80de\u4e2d (\u53e6\u4e24\u79cd\u4e3a\u6e38\u8d70\u5b62\u5b50\u53ca\u8425\u517b\u7ec6\u80de) \u7ec6\u80de\u58c1\u6700\u8584 (\u6216\u552f\u4e00\u7f3a\u4e4f\u7ec6\u80de\u58c1) \u7684\u4e00\u79cd\uff0c\u540c\u65f6\u5728\u7279\u5b9a\u57f9\u517b\u6761\u4ef6 (\u4f8b\u5982\u65bc\u57f9\u517b\u57fa\u5185\u6dfb\u52a00.9%\u786b\u9178\u94a0\u53d6\u4ee3\u6d77\u6c34\u7684\u4f5c\u6cd5) \u4ee5\u53ca\u7279\u5b9a\u57f9\u517b\u65f6\u95f4 (\u5bf9\u6570\u751f\u957f\u671f\uff1b\u63a5\u79cd12\u5c0f\u65f6\u524d\u5f8c) \u6709\u8f83\u9ad8\u6bd4\u4f8b\u7684\u963f\u7c73\u5df4\u7ec6\u80de\u51fa\u73b0 (\u5360\u603b\u7ec6\u80de\u4e4b16%)\uff0c\u53ef\u4f5c\u4e3a\u5229\u7528\u7535\u7a7f\u5b54\u6cd5\uff0c\u5c06\u5916\u6765\u57fa\u56e0\u6216\u53cc\u80a1RNA\u9001\u5165BL10\u7684\u80dc\u4efb\u7ec6\u80de\u6765\u4f7f\u7528\u3002
\n\u5f8c\u7eed\u4ee5\u6d41\u5f0f\u7ec6\u80de\u4eea\uff0c\u642d\u914d\u57fa\u56e0\u4f5312 Mbp\u7684\u9175\u6bcd\u83cc (Saccharomyces cerevisiae strain W303-1A) \u4f5c\u4e3a\u5185\u6807\u51c6\uff0c\u4ee5DNA\u7684\u76f8\u5bf9\u8424\u5149\u5f3a\u5ea6\uff0c\u6362\u7b97\u6240\u5f97\u4e4bBL10\u57fa\u56e0\u4f53\u5927\u5c0f\u4e3a40.5 Mbp\uff0c\u4ec5\u4e3aChlamydomonas (\u73b0\u884c\u5fae\u85fb\u6a21\u5f0f\u751f\u7269) \u76841\/3\u3002\u5e76\u4ee5\u8424\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u7ec6\u80de\u5206\u88c2\u4e2d\u671f\u7ec6\u80de\u7684\u67d3\u8272\u4f53\uff0c\u53d1\u73b0BL10\u5177\u67093\u6761\u67d3\u8272\u4f53\u7684\u6838\u578b\uff0c\u5e76\u4ee5\u8424\u5149\u539f\u4f4d\u6742\u4ea4\u8bc1\u5b9e\u4e3a\u5176\u5355\u5957\u751f\u7269\u4f53\uff0c\u8bf4\u660e\u6709\u673a\u4f1a\u80fd\u5229\u7528\u6b21\u4e16\u4ee3\u7684\u5b9a\u5e8f\u4eea\uff0c\u5b8c\u6210BL10\u5168\u57fa\u56e0\u7ec4\u7684\u5b9a\u5e8f\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"