{"id":3826,"date":"2016-05-18T11:11:01","date_gmt":"2016-05-18T03:11:01","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=3826"},"modified":"2016-05-18T11:11:01","modified_gmt":"2016-05-18T03:11:01","slug":"new-technique-2","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/new-technique-2.html","title":{"rendered":"\u7f8e\u56fd\u56fd\u5bb6\u5fae\u751f\u7269\u7ec4\u8ba1\u5212\u542f\u52a8\uff1a\u771f\u6b63\u7684\u65b0\u6280\u672f\u5728\u54ea\u91cc\uff1f"},"content":{"rendered":"

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<\/span> \u4f60\u4e0d\u77e5\u9053\u7684\u5fae\u85fb\u670b\u53cb<\/span><\/figcaption><\/figure>\n

 <\/p>\n

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\u6b64\u5916\uff0c2014\u5e74\uff0c\u7f8e\u56fd\u798f\u8d5b\u601d\u7814\u7a76\u6240\u3001\u9ebb\u7701\u7efc\u5408\u533b\u9662\uff08MGH\uff09\u548c\u54c8\u4f5b\u5927\u5b66\u516c\u5171\u536b\u751f\u5b66\u9662\u7b49\u5904\u7684\u79d1\u5b66\u5bb6\uff0c\u5f00\u53d1\u51fa\u4e00\u79cd\u65b0\u7684\u65b9\u6cd5\uff0c\u6536\u96c6\u7528\u4e8e\u57fa\u56e0\u7ec4\u548c\u8f6c\u5f55\u7ec4\u5206\u6790\u7684\u553e\u6db2\u4e0e\u7caa\u4fbf\u3002\u8be5\u65b9\u6cd5\u4e0d\u9700\u8981\u4e13\u4e1a\u4eba\u5458\u548c\u8bbe\u65bd\uff0c\u540c\u65f6\u8fd8\u80fd\u4fdd\u6301\u6837\u54c1\u7684\u5b8c\u6574\u6027\u3002<\/span><\/p>\n

\u7814\u7a76\u4eba\u5458\u8bbe\u8ba1\u4e86\u56fa\u5b9a\u5242\u7684\u9009\u62e9\u548c\u53d6\u6837\u6d41\u7a0b\uff08\u5982\u4e0b\u56fe\uff09\u3002\u53ef\u8ba98\u540d\u53d7\u8bd5\u8005\u5728\u5bb6\u91cc\u6536\u96c6\u5fae\u751f\u7269\u6837\u54c1\uff0c\u7136\u540e\u5c06\u5176\u8fd0\u81f3\u5b9e\u9a8c\u5ba4\u8fdb\u884c\u591a\u79cd\u7c7b\u578b\u7684\u5206\u5b50\u5206\u6790\u3002\u5c3d\u7ba1\u91c7\u7528\u4e0d\u540c\u7684\u53d6\u6837\u65b9\u6cd5\uff0c\u5fae\u751f\u7269\u79cd\u7c7b\u3001\u57fa\u56e0\u548c\u57fa\u56e0\u8f6c\u5f55\u7ec4\u6210\uff0c\u5728\u6240\u6709\u6837\u54c1\u4e2d\u90fd\u662f\u4e00\u81f4\u7684\u3002\u968f\u540e\u5bf9\u8fd9\u4e9b\u6837\u54c1\u8fdb\u884c\u7684\u5206\u6790\u8868\u660e\uff0c\u6d4b\u91cf\u7684\u4eba\u7c7b\u5fae\u751f\u7269\u7ec4\u7684\u529f\u80fd\u6f5c\u529b\u548c\u529f\u80fd\u6d3b\u6027\u4e4b\u95f4\u5b58\u5728\u7740\u6709\u8da3\u7684\u5f02\u540c\u70b9\u3002\u5728\u8fd9\u9879\u7814\u7a76\u4e2d\u662f\u76ee\u524d\u4e3a\u6b62\uff0c\u7ed3\u5408\u8868\u578b\u5206\u7c7b\u5b66\u3001\u5b8f\u57fa\u56e0\u7ec4\u5b66\u548c\u5b8f\u8f6c\u5f55\u7ec4\u6570\u636e\u8c31\uff0c\u8fdb\u884c\u7684\u7b2c\u4e00\u4e2a\u4eba\u7c7b\u5fae\u751f\u7269\u7ec4\u7814\u7a76\u3002\u7ed3\u679c\u8be6\u7ec6\u63cf\u8ff0\u4e86\u80a0\u9053\u83cc\u7fa4\u57fa\u56e0\u7ec4\u6f5c\u80fd\u548c\u57fa\u56e0\u8868\u8fbe\u4e4b\u95f4\u7684\u5173\u7cfb\uff0c\u9a8c\u8bc1\u4e86\u5728\u53d7\u8bd5\u8005\u6536\u96c6\u548c\u8fd0\u8f93\u7684\u6837\u54c1\u4e2d\u5f00\u5c55\u5b8f\u8f6c\u5f55\u7ec4\u7814\u7a76\u7684\u53ef\u884c\u6027\u3002<\/span><\/p>\n

\"\u53d6\u6837\u6d41\u7a0b\u56fe\"<\/a>
<\/span> \u53d6\u6837\u6d41\u7a0b\u56fe<\/span><\/figcaption><\/figure>\n

RNAlater\u6700\u521d\u662f\u4f5c\u4e3a\u4e00\u79cdRNA\u4fdd\u5b58\u5242\uff0c\u7531Qiagen\u548cThermo Fisher\u7b49\u5382\u5bb6\u5f00\u53d1\u51fa\u6765\u7684\uff08\u524d\u8005\u5f53\u5730\u4ef7\u683c\u4e3a\uff1a50mL\u768478\u7f8e\u5143\uff0c250mL\u7684324\u7f8e\u5143\uff1b\u540e\u8005\u5f53\u5730\u4ef7\u683c\u4e3a100mL\u7684125\u7f8e\u5143\uff0c500mL\u7684386\u7f8e\u5143\uff09\uff0c\u73b0\u5728\u53ef\u4ee5\u7528\u4e8e\u4fdd\u5b58DNA\u3002<\/span><\/p>\n

RNAlater\u662f\u7279\u6b8a\u7684\u6837\u54c1\u50a8\u5b58\u6db2\uff0c\u53ea\u8981\u5728\u91c7\u6837\u540e\u7acb\u5373\u5c06\u65b0\u9c9c\u6837\u54c1\u6d78\u5165\u8fd9\u79cd\u6db2\u4f53\u8bd5\u5242\uff0cRNlater\u53ef\u4ee5\u8fc5\u901f\u6e17\u900f\u5230\u7ec4\u7ec7\u6216\u5176\u4ed6\u751f\u7269\u6837\u672c\u4e2d\uff0c\u7a33\u5b9a\u5e76\u4fdd\u62a4RNA\u5b8c\u6574\u800c\u4e0d\u88ab\u964d\u89e3\uff0c\u786e\u4fdd\u4e0b\u6e38\u5206\u6790\u5f97\u5230\u7684\u6570\u636e\u771f\u5b9e\u53cd\u5e94\u6837\u54c1\u7684\u8868\u8fbe\u4fe1\u606f\u3002<\/span><\/p>\n

\u4eba\u7c7b\u5fae\u751f\u7269\u7ec4\u8ba1\u5212(Human Microbiome Project)\u7684\u53c2\u4e0e\u7814\u7a76\u4eba\u5458\uff0c\u6765\u81ea\u54c8\u4f5b\u5927\u5b66\u7684\u8ba1\u7b97\u751f\u7269\u5b66\u5bb6Curtis Huttenhower\u4e0e\u4ed6\u7684\u540c\u4e8b\u4eec\u53d1\u73b0\u5728\u6837\u54c1\u6536\u96c6\u540e\uff0c\u5982\u679c\u6ca1\u6709\u51b0\u7bb1\uff0c\u90a3\u4e48\u53ef\u4ee5\u52a0\u5165\u8fd9\u79cd\u4fdd\u62a4\u5242\u4f7f\u7528\u3002<\/span><\/p>\n

\u4e0d\u8fc7\u4e00\u4e9b\u7528\u6237\u4e5f\u62a5\u544a\u4e86RNAlater\u7684\u7f3a\u9677\uff0c\u867d\u7136RNAlater\u4e0d\u53ef\u71c3\uff0c\u56e0\u6b64\u53ef\u4ee5\u7528\u4e8e\u6837\u54c1\u8fd0\u8f93\uff0c\u4f46\u662f\u8fd9\u79cd\u4fdd\u62a4\u5242\u4f1a\u964d\u4f4eBacteroidetes \u548c Ruminococcaceae \u4e24\u79cd\u7ec6\u83cc\u65b0\u9c9c\u63d0\u53d6\u540e\u7684\u83cc\u7fa4\u6570\u91cf\u3002\u53e6\u5916\u5c31\u662f\u5982\u679c\u60f3\u83b7\u5f97\u6837\u54c1\u9ad8RNA\u4ea7\u91cf\uff0c\u90a3\u4e48\u5c31\u5fc5\u987b\u5148\u5c06\u5176\u53bb\u9664\u3002Ganz\u8868\u793a\uff0c\u201cRNAlater\u4f1a\u59a8\u788d\u6837\u54c1\u6b63\u5e38\u5f62\u6210\u9897\u7c92\u6c89\u6dc0\uff0c\u56e0\u6b64\u5728\u53bb\u9664RNAlater\u4e4b\u540e\uff0c\u8fd8\u5fc5\u987b\u78f7\u9178\u76d0\u7f13\u51b2\u6db2\u8fdb\u884c\u6e05\u6d17\u3002\u201d <\/span><\/p>\n

\u6b64\u5916\uff0cWoods Hole\u6d77\u6d0b\u7814\u7a76\u6240\u6d77\u6d0b\u751f\u7269\u5316\u5b66\u5bb6Mak Saito\u4e5f\u6307\u51fa\uff0cRNAlater\u8fd8\u6709\u4e00\u4e2a\u95ee\u9898\uff0c\u90a3\u5c31\u662f\u5176\u9ad8\u76d0\u542b\u91cf\uff0c\u56e0\u6b64\u5728\u4e00\u4e9b\u5b9e\u9a8c\u4e2d\u4f1a\u4ee4\u6837\u54c1\u6e05\u6d17\u6210\u4e3a\u95ee\u9898\u3002Saito\u66fe\u91c7\u7528\u8fd9\u79cd\u6eb6\u6db2\u4fdd\u5b58\u6d77\u6d0b\u5fae\u751f\u7269\u7ec4\u4e2d\u7684\u86cb\u767d\u7ec4\u6210\u3002<\/span><\/p>\n

\u5bf9\u4e8e\u4e00\u4e9b\u7c7b\u578b\u7684\u5b9e\u9a8c\u6837\u54c1\u6765\u8bf4\uff0c\u5982\u679c\u52a0\u5165\u4f4e\u6e29\u51bb\u5b58\u4fdd\u62a4\u5242\u5c31\u4f1a\u5927\u5e45\u5ea6\u6539\u53d8\u5fae\u751f\u7269\u7ec4\u6210\uff0c\u8fd9\u6837\u505a\u5212\u4e0d\u6765\uff0c\u6bd4\u5982\u571f\u58e4\u6837\u54c1\u3002\u800c\u5bf9\u7caa\u4fbf\u6837\u54c1\u800c\u8a00\uff0c\u6709\u65f6\u4f4e\u6e29\u51bb\u5b58\u4e5f\u8bb8\u5e76\u4e0d\u5408\u9002\uff0c\u6bd4\u5982\u8bf4\uff0c\u7528\u4e8e\u6cbb\u7597\u8270\u96be\u68ad\u83cc\u611f\u67d3\u7684\u6837\u54c1\u80fd\u5728\u5ba4\u6e29\u4e0b\u4fdd\u5b58\u957f\u8fbe6\u4e2a\u5c0f\u65f6\uff0c\u800c\u4e14\u8fd8\u4e0d\u4f1a\u5f15\u8d77\u6cbb\u7597\u7597\u6548\u53d1\u751f\u663e\u8457\u53d8\u5316\u3002<\/span><\/p>\n

\u6d4b\u5e8f\u6280\u672f<\/strong><\/span><\/p>\n

\u9ad8\u901a\u91cf\u57fa\u56e0\u6d4b\u5e8f\u6280\u672f\u65e0\u7591\u662f\u5fae\u751f\u7269\u7ec4\u7814\u7a76\u7684\u4e00\u5927\u4f9d\u9760\uff0c\u4f46\u662f\u5bf9\u4e8e\u7eb7\u7e41\u590d\u6742\u7684\u5fae\u751f\u7269\u7ec4\u6765\u8bf4\uff0c\u6d4b\u5e8f\u6280\u672f\u5de5\u5177\u8fd8\u9700\u4e0d\u65ad\u7684\u5f00\u53d1\u6539\u8fdb\uff0c\u53bb\u5e74\u5e74\u5e95\uff0c\u65af\u5766\u798f\u5927\u5b66\u7684\u7814\u7a76\u4eba\u5458\u9996\u6b21\u5f00\u53d1\u51fa\u4e86\u4e00\u79cd\u65b0\u6d4b\u5e8f\u6280\u672f\uff0c\u7528\u4e8e\u5206\u6790\u4eba\u7c7b\u80a0\u9053\u5fae\u751f\u7269\u7ec4\uff0c\u514b\u670d\u4e86\u77ed\u8bfb\u53d6\u6d4b\u5e8f\u7684\u6280\u672f\u9650\u5236\u3002<\/span><\/p>\n

\u4ed6\u4eec\u5c06Illumina\u957f\u8bfb\u53d6\u6280\u672f\uff08TruSeq Synthetic Long-Reads\uff09\u4e0e\u8ba1\u7b97\u5de5\u5177\u7ed3\u5408\u8d77\u6765\uff0c\u514b\u670d\u4e86\u77ed\u8bfb\u53d6\u6d4b\u5e8f\u7684\u6280\u672f\u9650\u5236\uff0c\u83b7\u5f97\u4e86\u4e00\u540d\u7537\u6027\u80a0\u9053\u5fae\u751f\u7269\u7ec4\u7684\u6e05\u6670\u56fe\u8c31\u3002<\/span><\/p>\n

\u76ee\u524d\u901a\u5e38\u662f\u901a\u8fc7\u77ed\u8bfb\u53d6\u6d4b\u5e8f\u83b7\u5f97\u4e00\u5c0f\u6bb5\u4e00\u5c0f\u6bb5\u7684\u4fe1\u606f\uff0c\u4e3a\u4e86\u6539\u5584\u8fd9\u4e00\u95ee\u9898\uff0c\u7814\u7a76\u4eba\u5458\u91c7\u7528\u65b0\u65b9\u6cd5\uff0c\u5c06\u8f83\u5927\u7684\u57fa\u56e0\u7ec4\u7247\u6bb5\u88c5\u8ba2\u5728\u4e00\u8d77\u3002\u9996\u5148\u7528\u590d\u6742\u7684\u7b97\u6cd5\u5148\u5c06\u5c0f\u7247\u6bb5\u7ec4\u88c5\u621010,000-base\u7247\u6bb5\uff0c\u518d\u5c0610,000-base\u7247\u6bb5\u7ec4\u88c5\u6210\u66f4\u957f\u7684\u7247\u6bb5\uff0c\u7136\u540e\u62fc\u51fa\u6574\u4e2a\u57fa\u56e0\u7ec4\u3002\u7814\u7a76\u6307\u51fa\uff0c\u5c06Illumina\u957f\u8bfb\u53d6\u6280\u672f\u4e0e\u77ed\u8bfb\u53d6\u6d4b\u5e8f\u76f8\u7ed3\u5408\uff0c\u80fd\u591f\u66f4\u52a0\u7cbe\u786e\u548c\u5168\u9762\u7684\u5206\u6790\u5b8f\u57fa\u56e0\u7ec4\u6837\u672c\uff0c\u66f4\u5bb9\u6613\u5728\u4eba\u3001\u52a8\u7269\u3001\u690d\u7269\u3001\u6c34\u548c\u571f\u58e4\u4e2d\u5efa\u7acb\u81f4\u75c5\u83cc\u83cc\u682a\u7684\u8fdb\u5316\u53f2\u3002<\/span><\/p>\n

\u4f46\u662f\u76ee\u524d\u4e00\u4e9b\u79d1\u5b66\u5bb6\u4e5f\u63d0\u51fa\uff0cDNA\u63d0\u53d6\u8bd5\u5242\u76d2\u3001\u5316\u5b66\u8bd5\u5242\u548c\u5b9e\u9a8c\u5ba4\u73af\u5883\u4e2d\u7684\u6742\u83cc\u5f88\u5bb9\u6613\u9020\u6210\u6c61\u67d3\uff0c\u5f71\u54cd\u5fae\u751f\u7269\u7ec4\u5206\u6790\u7684\u7ed3\u679c\u3002 \u00a0\u7814\u7a76\u4eba\u5458\u53d1\u73b0\uff0c\u6ca1\u6709\u6c61\u67d3\u7684\u8bdd\u5bf9\u7167\u6837\u672c\u5e94\u8be5\u53ea\u6709\u4e00\u79cd\u83cc\uff0c\u4f46\u6709\u65f6\u5374\u51fa\u73b0\u4e86270\u79cd\u4e0d\u540c\u7684\u7ec6\u83cc\u3002\u4e0e\u9ad8\u751f\u7269\u91cf\u7684\u6837\u672c\u76f8\u6bd4\uff08\u7caa\u4fbf\u6837\u672c\uff09\uff0c\u6765\u81ea\u8840\u6db2\u6216\u80ba\u90e8\u7684\u4f4e\u751f\u7269\u91cf\u6837\u672c\u5c24\u5176\u5bb9\u6613\u53d7\u5230\u6c61\u67d3\u3002<\/span><\/p>\n

\u4e3a\u6b64\uff0c\u7814\u7a76\u4eba\u5458\u5efa\u8bae\u7814\u7a76\u8005\u4eec\u5728\u7814\u7a76\u4f4e\u751f\u7269\u91cf\u6837\u672c\u65f6\u91c7\u53d6\u4e00\u4e9b\u989d\u5916\u7684\u6b65\u9aa4\u3002\u6bd4\u5982\u4f7f\u7528\u9634\u6027\u5bf9\u7167\u9274\u5b9a\u53ef\u80fd\u6c61\u67d3\u6837\u672c\u7684\u6742\u83cc\uff0c\u8fdb\u884c\u8fc7\u6ee4\u6216\u5bcc\u96c6\u4ee5\u589e\u5927\u521d\u59cb\u6750\u6599\u4e2d\u7684\u751f\u7269\u91cf\uff0c\u5728\u6837\u672c\u6536\u96c6\u7684\u65f6\u5019\u5c3d\u91cf\u964d\u4f4e\u6c61\u67d3\u7684\u53ef\u80fd\u3002<\/span><\/p>\n

\u4fe1\u606f\u5206\u6790\u5de5\u5177<\/strong><\/span><\/p>\n

\u5728\u5206\u79bb\u548c\u6d4b\u5e8f\u7ec6\u83cc\u6838\u9178\u4e4b\u540e\uff0c\u6211\u4eec\u9700\u8981\u4ece\u6570\u636e\u4e2d\u63d0\u53d6\u6709\u6548\u7684\u4fe1\u606f\uff0c\u8fd9\u5e76\u4e0d\u662f\u4e00\u4ef6\u5bb9\u6613\u7684\u4e8b\u513f\u3002\u5fae\u751f\u7269\u7ec4\u7814\u7a76\u8005\u4e00\u822c\u6709\u4e24\u4e2a\u9009\u62e9\uff1a16S rDNA\u6d4b\u5e8f\u548c\u5b8f\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u300216S rDNA\u6d4b\u5e8f\u53ef\u4ee5\u5c55\u73b0\u6837\u672c\u4e2d\u7684\u5fae\u751f\u7269\u79cd\u7c7b\u548c\u4e30\u5ea6\uff0c\u53cd\u6620\u5fae\u751f\u7269\u591a\u6837\u6027\u7684\u5927\u89c4\u6a21\u6539\u53d8\u3002\u800c\u5b8f\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u53ef\u4ee5\u63ed\u793a\u5fae\u751f\u7269\u7fa4\u4f53\u7684\u4ee3\u8c22\u6f5c\u80fd\uff0c\u5e2e\u52a9\u4eba\u4eec\u7ec4\u88c5\u4e0d\u540c\u5fae\u751f\u7269\u7684\u57fa\u56e0\u7ec4\u3002<\/span><\/p>\n

16S rRNA\u5206\u6790\u6700\u5e38\u7528\u7684\u5de5\u5177\u662f\u5f00\u6e90\u7684QIIME\u548cMothur\u3002\u5f53\u7136\u5e02\u9762\u4e0a\u4e5f\u6709\u5546\u4e1a\u5316\u7684\u7a0b\u5e8f\u5305\uff0c\u6bd4\u5982Thermo Fisher\u516c\u53f8\u7684Ion 16S Metagenomics Kit\u3002\u8fd9\u662f\u4e00\u4e2a\u57fa\u4e8e\u7f51\u9875\u548c\u4e91\u7aef\u7684\u514d\u8d39\u7a0b\u5e8f\u5305\u3002\u8fc7\u53bb\u7684\u5fae\u751f\u7269\u7ec4\u7814\u7a76\u4e3b\u8981\u4f7f\u752816S rDNA\u6d4b\u5e8f\u3002\u968f\u7740\u6d4b\u5e8f\u6210\u672c\u7684\u76f4\u7ebf\u4e0b\u964d\uff0c\u9488\u5bf9\u6574\u4e2a\u83cc\u7fa4\u7684\u5b8f\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u8d8a\u6765\u8d8a\u53d7\u5230\u9752\u7750\uff0c\u56e0\u4e3a\u8fd9\u4e00\u6280\u672f\u80fd\u591f\u63d0\u4f9b\u66f4\u5168\u9762\u7684\u57fa\u56e0\u7ec4\u548c\u529f\u80fd\u4fe1\u606f\u3002<\/span><\/p>\n

\u54c8\u4f5b\u5927\u5b66\u516c\u5171\u536b\u751f\u5b66\u9662\u7684Eric Franzosa\u4e3a\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u5206\u6790\u5f00\u53d1\u4e86HUMAnN2\u3002\u7b2c\u4e00\u4ee3HUMAnN\u4e3b\u8981\u662f\u6839\u636e\u5b8f\u57fa\u56e0\u7ec4\u6216\u8005\u5b8f\u8f6c\u5f55\u7ec4\u6570\u636e\uff0c\u5206\u6790\u5fae\u751f\u7269\u901a\u8def\u5728\u7fa4\u4f53\u4e2d\u662f\u5426\u5b58\u5728\u3002\u4e5f\u5c31\u662f\u8bf4\uff0c\u7ed9HUMAnN\u63d0\u4f9b\u5fae\u751f\u7269\u7fa4\u843d\u7684DNA\u6216RNA\u5e8f\u5217\uff0c\u5b83\u5c31\u4f1a\u544a\u8bc9\u4f60\u8fd9\u4e2a\u7fa4\u4f53\u80fd\u591f\u6267\u884c\u4ec0\u4e48\u6837\u7684\u5206\u5b50\u529f\u80fd\u3002HUMAnN2\u5728HUMAnN\u7684\u57fa\u7840\u4e0a\u8fdb\u884c\u4e86\u62d3\u5c55\uff0c\u5c06\u5fae\u751f\u7269\u4e0e\u7279\u5b9a\u6d3b\u6027\u5173\u8054\u8d77\u6765\u3002<\/span><\/p>\n

MetaPath\u4e5f\u662f\u4e00\u4e2a\u7528\u4e8e\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u5206\u6790\u7684\u91cd\u8981\u5de5\u5177\u3002\u8be5\u5de5\u5177\u7531\u9a6c\u91cc\u5170\u5927\u5b66\u7684\u526f\u6559\u6388Mihai Pop\u5f00\u53d1\uff0c\u53ef\u4ee5\u6839\u636e\u5b8f\u57fa\u56e0\u7ec4\u6570\u636e\u5206\u6790\u7279\u5b9a\u7fa4\u4f53\u5728\u4e0d\u540c\u6761\u4ef6\u4e0b\u7684\u4ee3\u8c22\u53d8\u5316\u3002\u4e3e\u4f8b\u6765\u8bf4\uff0cPop\u7684\u7814\u7a76\u56e2\u961f\u5bf9\u80d6\u4eba\u548c\u7626\u4eba\u8fdb\u884c\u5fae\u751f\u7269\u7ec4\u7814\u7a76\uff0c\u901a\u8fc7MetaPath\u9274\u5b9a\u4e86\u8102\u80aa\u9178\u751f\u7269\u5408\u6210\u901a\u8def\u7684\u5dee\u5f02\u3002<\/span><\/p>\n

\u5b8f\u57fa\u56e0\u7ec4\u7814\u7a76\u901a\u5e38\u662f\u4ece\u73af\u5883\u4e2d\u6536\u96c6\u5fae\u751f\u7269\u548c\u75c5\u6bd2\u6837\u54c1\uff0c\u7136\u540e\u5c06\u8fd9\u4e9b\u6837\u672c\u7834\u788e\uff0c\u628a\u5b83\u4eec\u7684\u57fa\u56e0\u7ec4DNA\u964d\u89e3\u6210\u7247\u6bb5\uff0c\u6700\u540e\u901a\u8fc7\u6d4b\u5e8f\u4eea\u8fdb\u884c\u5206\u6790\u3002\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u6bd4\u4e00\u822c\u7684\u57fa\u56e0\u7ec4\u5206\u6790\u9700\u8981\u66f4\u591a\u7684\u501f\u52a9\u8ba1\u7b97\u673a\u6280\u672f\uff0c\u56e0\u4e3a\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u5904\u7406\u7684\u662f\u4e0d\u540c\u57fa\u56e0\u7ec4\u7684\u6df7\u5408\u7269\uff0c\u800c\u4e0d\u662f\u5355\u7eaf\u7684\u540c\u8d28\u5fae\u751f\u7269\u79cd\u7fa4\u3002\u5b8f\u57fa\u56e0\u7ec4\u5206\u6790\u4ea7\u751f\u7684\u6570\u636e\u6bd4\u4e00\u822c\u57fa\u56e0\u7ec4\u5206\u6790\u591a\u5f97\u591a\uff0c\u8fd9\u662f\u8fd9\u4e00\u7814\u7a76\u9886\u57df\u9762\u4e34\u7684\u4e00\u5927\u6311\u6218\u3002 <\/span><\/p>\n

\u5e38\u7528\u7684\u4e00\u4e9b\u5de5\u5177\uff0c\u5982MEGAN5\u4e3b\u8981\u7528\u4e8eMEtaGenome ANalyzer\uff0c\u6700\u521d\u662f\u57282007\u5e74\u5f00\u53d1\u51fa\u6765\uff0c\u7528\u4e8e\u8bc6\u522b\u957f\u6bdb\u731b\u72b8\u8c61\u9aa8\u4e2dDNA\u6d4b\u5e8f\u7814\u7a76\u7684\u5fae\u751f\u7269\u6c61\u67d3\u3002MEGAN5\u662f\u53d1\u5e03\u7684\u6700\u65b0\u7248\u672c\uff0c\u9664\u4e86\u53ef\u4ee5\u5e2e\u52a9\u8fdb\u884c\u5206\u7c7b\u5206\u6790\uff0c\u4e5f\u53ef\u4ee5\u5feb\u901f\u6bd4\u8f83\u591a\u4e2a\u6570\u636e\u96c6\uff0c\u533a\u5206\u5b8f\u57fa\u56e0\u7ec4\u4e2d\u57fa\u56e0\u7684\u529f\u80fd\uff0c\u6b64\u5916\u8fd8\u63d0\u4f9b\u5143\u6570\u636emetadata\u652f\u6301\u548c\u6570\u636e\u53ef\u89c6\u5316\u7684\u65b0\u9014\u5f84\u3002<\/span><\/p>\n

\u6b64\u5916\uff0c\u8fd1\u671fNature Methods\u6742\u5fd7\u53d1\u5e03\u4e86\u4e00\u4e2a\u540d\u4e3aTruSPADES\u7684\u5f3a\u5927\u5de5\u5177\uff0c\u53ef\u4ee5\u5927\u5927\u63d0\u5347\u7814\u7a76\u8005\u4eec\u6d4b\u5e8f\u5b8f\u57fa\u56e0\u7ec4\u7684\u80fd\u529b\u3002\u8fd9\u79cd\u7b97\u6cd5\u80fd\u5c06Illumina\u6d4b\u5e8f\u4eea\u751f\u6210\u7684300bp\u77ed\u8bfb\u53d6\uff0c\u5408\u5e76\u6210\u5927\u7ea61\u4e07bp\u7684\u57fa\u56e0\u7ec4\u7247\u6bb5\uff08Synthetic Long Reads\uff09\u3002\u5982\u679c\u8bf4\u7528\u77ed\u8bfb\u53d6\u76f8\u5f53\u4e8e\u8bed\u53e5\uff0c\u90a3\u4e48\u957f\u7247\u6bb5\u5c31\u662f\u6574\u7ae0\u6587\u5b57\u3002\u663e\u800c\u6613\u89c1\uff0c\u628a\u6574\u7ae0\u6587\u5b57\u8fd8\u539f\u6210\u4e00\u672c\u4e66\u8981\u5bb9\u6613\u5f97\u591a\u3002<\/span><\/p>\n

TruSPADES\u4e3b\u8981\u662f\u5148\u7ed9100-300bp\u7684\u77ed\u8bfb\u53d6\u88c5\u4e0a\u6761\u7801\uff0c\u7136\u540e\u901a\u8fc7de brujin \u56fe\u628a\u8fd9\u4e9b\u7247\u6bb5\u7ec4\u88c5\u8d77\u6765\uff0c\u751f\u6210Synthetic Long Reads\u3002\u8fd9\u79cd\u4f4e\u6210\u672c\u7684\u65b9\u6cd5\u53ef\u4ee5\u66f4\u597d\u7684\u786e\u5b9a\u76f8\u8fde\u7247\u6bb5\uff0c\u83b7\u5f97\u66f4\u957f\u66f4\u51c6\u786e\u7684\u957f\u6d4b\u5e8f\u7247\u6bb5\u3002<\/span><\/p>\n

\u9898\u5916\u8bdd\uff1a<\/span><\/p>\n

\u867d\u7136\u5fae\u751f\u7269\u7ec4\u70ed\u6f6e\u94fa\u5929\u76d6\u5730\uff0c\u4f46\u662f\u4e00\u4e9b\u79d1\u5b66\u5bb6\u8ba4\u4e3a\u8fd9\u5176\u5b9e\u63a9\u76d6\u4e86\u5176\u4e2d\u7684\u98ce\u9669\u3002\u56de\u987e\u4e4b\u524d\u7684\u79cd\u79cd\u201c\u7ec4\u5b66\u201d\u70ed\u6211\u4eec\u53ef\u4ee5\u770b\u5230\uff0c\u6280\u672f\u8fdb\u6b65\u8d4b\u4e88\u4e86\u4eba\u4eec\u5206\u6790\u57fa\u56e0\u7ec4\u3001\u86cb\u767d\u8d28\u7ec4\u3001\u4ee3\u8c22\u7ec4\u7684\u80fd\u529b\uff0c\u4e8e\u662f\u4fbf\u6709\u5927\u91cf\u7814\u7a76\u5c06\u8fd9\u4e9b\u5206\u5b50\u7684\u72b6\u6001\u4e0e\u4eba\u4f53\u5065\u5eb7\u8054\u7cfb\u8d77\u6765\u3002\u7136\u800c\uff0c\u8fd9\u4e9b\u7814\u7a76\u7684\u540e\u7eed\u5de5\u4f5c\u5f80\u5f80\u540e\u7ee7\u65e0\u529b\uff0c\u60c5\u51b5\u53cd\u800c\u8d8a\u6765\u8d8a\u590d\u6742\u3002<\/span><\/p>\n

\u6b64\u524dNature\u6742\u5fd7\u66fe\u7ecf\u520a\u6587\uff0c\u5bf9\u5fae\u751f\u7269\u7ec4\u70ed\u6f6e\u8fdb\u884c\u4e86\u53cd\u601d\u3002\u6587\u7ae0\u6307\u51fa\uff0c\u5728\u63a5\u53d7\u4e00\u9879\u65b0\u7ed3\u8bba\u4e4b\u524d\uff0c\u6211\u4eec\u5e94\u8be5\u9996\u5148\u63d0\u51fa\u4e94\u4e2a\u95ee\u9898\uff1a<\/span><\/p>\n

\u7814\u7a76\u6709\u6ca1\u6709\u68c0\u6d4b\u5230\u6709\u610f\u4e49\u7684\u5dee\u5f02\uff1f<\/strong><\/span><\/p>\n

\u5fae\u751f\u7269\u7ec4\u56fe\u8c31\u4e3b\u8981\u4f9d\u8d5616S rRNA\u5206\u6790\uff0c\u80fd\u63d0\u4f9b\u95e8\u3001\u5c5e\u3001\u79cd\u7684\u4fe1\u606f\uff0c\u4f46\u8fd9\u79cd\u5206\u7c7b\u8fd8\u4e0d\u591f\u7ec6\u3002\u4e3e\u4f8b\u6765\u8bf4\uff0c\u6709\u7814\u7a76\u9274\u5b9a\u4e86\u4e0e\u7cd6\u5c3f\u75c5\u6709\u5173\u7684\u5fae\u751f\u7269\u7ec4\uff0c\u662f\u7ec6\u83cc\u4e0d\u540c\u95e8\u4e4b\u95f4\u5448\u7279\u5b9a\u6bd4\u4f8b\uff0c\u8fd9\u672a\u514d\u592a\u8fc7\u4e8e\u5bbd\u6cdb\u3002\u8fd9\u76f8\u5f53\u4e8e\u662f\u8ba4\u4e3a100\u53ea\u9e1f\u548c25\u53ea\u8717\u725b\u7ec4\u6210\u7684\u9e1f\u820d\u7b49\u540c\u4e8e\u67098\u6761\u9c7c\u548c2\u53ea\u4e4c\u8d3c\u7684\u6c34\u7bb1\uff0c\u53ea\u56e0\u4e3a\u9e1f\u820d\u548c\u6c34\u7bb1\u91cc\u7684\u810a\u690e\u52a8\u7269\u548c\u8f6f\u4f53\u52a8\u7269\u4e4b\u6bd4\u90fd\u662f4:1\u3002\u53e6\u5916\uff0c\u5c31\u7b97\u662f\u540c\u4e00\u4e2a\u5c5e\u7684\u5fae\u751f\u7269\uff0c\u6240\u542b\u57fa\u56e0\u4e5f\u5f80\u5f80\u5927\u76f8\u5f84\u5ead\u3002<\/span><\/p>\n

\u5982\u4eca\u7684\u6280\u672f\u5176\u5b9e\u53ef\u4ee5\u505a\u5230\u66f4\u7ec6\u81f4\u7684\u5206\u7c7b\uff0c\u6211\u4eec\u53ef\u4ee5\u901a\u8fc7\u7834\u8bd1\u201c\u4ee3\u8c22\u7f51\u7edc\u201d\u6765\u7406\u89e3\u5fae\u751f\u7269\u7ec4\u8fdb\u884c\u7684\u751f\u5316\u53cd\u5e94\uff0c\u5e76\u5728\u6b64\u57fa\u7840\u4e0a\u9274\u5b9a\u5bf9\u5065\u5eb7\u6709\u5f71\u54cd\u7684\u57fa\u56e0\u7ec4\u5408\u3002\u4e0d\u8fc7\u8fd9\u5c31\u8981\u6c42\u7814\u7a76\u8005\u5bf9\u6574\u4e2a\u7f51\u7edc\u6709\u4e00\u4e2a\u900f\u5f7b\u7684\u4e86\u89e3\u3002<\/span><\/p>\n

\u7814\u7a76\u662f\u5426\u660e\u786e\u4e86\u56e0\u679c\u5173\u7cfb\uff1f<\/strong><\/span><\/p>\n

\u5728\u9274\u5b9a\u75be\u75c5\u76f8\u5173\u5fae\u751f\u7269\u7ec4\u65f6\uff0c\u6211\u4eec\u9700\u8981\u786e\u5b9a\u8fd9\u79cd\u5173\u7cfb\u5230\u5e95\u662f\u4e0d\u662f\u56e0\u679c\u5173\u7cfb\uff0c\u56e0\u4e3a\u8fd9\u79cd\u7279\u522b\u7684\u5fae\u751f\u7269\u7ec4\u6709\u65f6\u53ef\u80fd\u53ea\u662f\u4e2a\u526f\u4ea7\u7269\u3002 <\/span><\/p>\n

2012\u5e74\u6709\u4e00\u9879\u7814\u7a76\u53d1\u73b0\uff0c\u4f4f\u5728\u517b\u8001\u9662\u91cc\u7684\u8001\u4eba\u5fae\u751f\u7269\u7ec4\u4e0e\u4f4f\u5728\u793e\u533a\u91cc\u7684\u8001\u4eba\u4e0d\u540c\uff0c\u800c\u4e14\u8fd9\u79cd\u5dee\u5f02\u4e0e\u4f53\u8d28\u5f3a\u5f31\u3002\u4f5c\u8005\u5728\u8003\u8651\u4e86\u591a\u79cd\u56e0\u7d20\u540e\u6307\u51fa\uff0c\u996e\u98df\u80fd\u591f\u6539\u53d8\u5fae\u751f\u7269\u7ec4\u8fdb\u800c\u5f71\u54cd\u5065\u5eb7\u72b6\u51b5\u3002\u8fd9\u79cd\u7ed3\u8bba\u7684\u786e\u4e0e\u6570\u636e\u76f8\u7b26\uff0c\u4f46\u7814\u7a76\u8005\u5374\u6ca1\u80fd\u53cd\u5411\u8bc1\u660e\u8fd9\u6837\u7684\u56e0\u679c\u5173\u7cfb\u3002\u56e0\u4e3a\u53ef\u80fd\u5f71\u54cd\u5fae\u751f\u7269\u7ec4\u7684\u56e0\u7d20\u5f88\u591a\uff0c\u6bd4\u5982\u514d\u75ab\u7cfb\u7edf\u6bd4\u8f83\u4e0d\u6d3b\u8dc3\uff0c\u6d88\u5316\u80fd\u529b\u8f83\u5f31\u7b49\u7b49\u3002<\/span><\/p>\n

\u4f5c\u7528\u673a\u5236\u662f\u4ec0\u4e48\uff1f<\/strong><\/span><\/p>\n

\u6211\u4eec\u90fd\u77e5\u9053\uff0c\u76f8\u5173\u6027\u5e76\u4e0d\u7b49\u540c\u4e8e\u56e0\u679c\u5173\u7cfb\u3002\u4e0d\u8fc7\u5b58\u5728\u76f8\u5173\u6027\uff0c\u591a\u534a\u610f\u5473\u7740\u67d0\u79cd\u56e0\u679c\u5173\u7cfb\u7684\u5b58\u5728\uff0c\u8fd9\u9700\u8981\u6211\u4eec\u52aa\u529b\u53bb\u53d1\u6398\u3002<\/span><\/p>\n

\u73b0\u5728\uff0c\u7814\u7a76\u8005\u4eec\u53ef\u4ee5\u901a\u8fc7\u8bbe\u8ba1\uff0c\u7cbe\u786e\u9650\u5b9a\u5fae\u751f\u7269\u7ec4\u4e2d\u7684\u6210\u5206\uff0c\u5efa\u7acb\u7f3a\u4e4f\u67d0\u4e00\u7c7b\u5fae\u751f\u7269\u7684\u83cc\u7fa4\uff0c\u6216\u8005\u5229\u7528\u201c\u82af\u7247\u4e0a\u7684\u5668\u5b98\u201d\u6d4b\u5b9a\u5fae\u751f\u7269\u7ec4\u7684\u751f\u5316\u6d3b\u6027\u3002\u8fd9\u6837\u7684\u65b9\u6cd5\u80fd\u591f\u5e2e\u52a9\u6211\u4eec\u786e\u5b9a\uff0c\u67d0\u4e00\u79cd\u5fae\u751f\u7269\u7ec4\u5bf9\u4eba\u4f53\u5065\u5eb7\u7684\u5f71\u54cd\u3002<\/span><\/p>\n

\u7814\u7a76\u662f\u5426\u8db3\u4ee5\u53cd\u6620\u771f\u5b9e\u60c5\u51b5\uff1f<\/strong><\/span><\/p>\n

\u4e0d\u8fc7\uff0c\u5c31\u7b97\u5fae\u751f\u7269\u7ec4\u5728\u5b9e\u9a8c\u4e2d\u80fd\u591f\u5f71\u54cd\u5065\u5eb7\uff0c\u4e5f\u53ef\u80fd\u5e76\u4e0d\u662f\u9020\u6210\u60a3\u8005\u75c7\u72b6\u7684\u4e3b\u8981\u539f\u56e0\u3002<\/span><\/p>\n

\u4e3e\u4f8b\u6765\u8bf4\uff0c\u5fae\u751f\u7269\u7ec4\u7814\u7a76\u5f80\u5f80\u4f7f\u7528\u65e0\u83cc\u5c0f\u9f20\uff0c\u4ee5\u4fbf\u5f15\u5165\u5b9e\u9a8c\u6027\u7684\u83cc\u7fa4\u3002\u4f46\u662f\u8fd9\u4e9b\u5c0f\u9f20\u5e76\u4e0d\u80fd\u4ee3\u8868\u52a8\u7269\u7684\u5929\u7136\u72b6\u6001\uff0c\u7f3a\u4e4f\u5fae\u751f\u7269\u7ec4\u7684\u5b83\u4eec\u5176\u5b9e\u5e76\u4e0d\u5065\u5eb7\u3002\u56e0\u6b64\uff0c\u5b9e\u9a8c\u7ed3\u679c\u5e76\u4e0d\u4e00\u5b9a\u7b26\u5408\u52a8\u7269\u7684\u5929\u7136\u5e94\u7b54\u3002\u53e6\u5916\uff0c\u5c0f\u9f20\u7684\u751f\u6d3b\u73af\u5883\u4e0e\u4eba\u7c7b\u5dee\u5f02\u5f88\u5927\uff0c\u6709\u5173\u5fae\u751f\u7269\u7ec4\u7684\u5b9e\u9a8c\u7ed3\u679c\u4e5f\u8bb8\u5e76\u4e0d\u80fd\u5f88\u597d\u7684\u7528\u4e8e\u4eba\u7c7b\u3002<\/span><\/p>\n

\u7ed3\u679c\u662f\u5426\u53ef\u80fd\u6709\u522b\u7684\u89e3\u91ca\uff1f\u6211\u4eec\u6709\u5145\u5206\u7684\u7406\u7531\u76f8\u4fe1\uff0c\u7ec6\u83cc\u6b63\u5728\u901a\u8fc7\u591a\u79cd\u9014\u5f84\u5bf9\u6211\u4eec\u65bd\u52a0\u5f71\u54cd\u3002\u4f46\u662f\u4e5f\u6709\u8bb8\u591a\u5176\u4ed6\u56e0\u7d20\uff08\u53ef\u80fd\u66f4\u91cd\u8981\uff09\u5728\u8d77\u4f5c\u7528\uff0c\u6bd4\u5982\u996e\u98df\u3002\u5728\u628a\u67d0\u79cd\u5fae\u751f\u7269\u7ec4\u4e0e\u75be\u75c5\u5173\u8054\u8d77\u6765\u4e4b\u524d\uff0c\u660e\u667a\u7684\u505a\u6cd5\u662f\uff0c\u628a\u6240\u6709\u53ef\u80fd\u7684\u56e0\u7d20\u90fd\u7eb3\u5165\u8003\u8651\u3002<\/span><\/p>\n

\u53c2\u8003\u6587\u732e\uff1a<\/span><\/p>\n

Methods for Improving Human Gut Microbiome Data by Reducing Variability through Sample Processing and Storage of Stool<\/span><\/p>\n

Relating the metatranscriptome and metagenome of the human gut <\/span><\/p>\n

Examination of Microbial Proteome Preservation Techniques Applicable to Autonomous Environmental Sample Collection<\/span><\/p>\n

TruSPAdes: barcode assembly of TruSeq synthetic long reads <\/span><\/p>\n

Synthetic long-read sequencing reveals intraspecies diversity in the human microbiome <\/span><\/p>\n

Reagent and laboratory contamination can critically impact sequence-based microbiome analyses<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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