{"id":4951,"date":"2017-04-20T11:38:59","date_gmt":"2017-04-20T03:38:59","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=4951"},"modified":"2017-04-20T11:38:59","modified_gmt":"2017-04-20T03:38:59","slug":"cyanobacteria-ethanol","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/cyanobacteria-ethanol.html","title":{"rendered":"\u9752\u5c9b\u80fd\u6e90\u6240\u5728\u84dd\u7ec6\u83cc\u5149\u5408\u751f\u7269\u5408\u6210\u4e59\u9187\u65b9\u9762\u53d6\u5f97\u7cfb\u5217\u8fdb\u5c55"},"content":{"rendered":"

\u4e59\u9187\u662f\u751f\u4ea7\u89c4\u6a21\u6700\u5927\u3001\u5e94\u7528\u7a0b\u5ea6\u6700\u9ad8\u7684\u53ef\u518d\u751f\u751f\u7269\u6db2\u4f53\u71c3\u6599\u3002\u73b0\u9636\u6bb5\uff0c\u751f\u7269\u4e59\u9187\u7684\u4e3b\u8981\u6765\u6e90\u662f\u91c7\u7528\u542b\u7cd6\u91cf\u4e30\u5bcc\u7684\u519c\u4e1a\u751f\u7269\u8d28\u4e3a\u539f\u6599\u7684\u751f\u7269\u70bc\u5236\u8fc7\u7a0b\uff0c\u4ee5\u201c\u7389\u7c73\u4e59\u9187\u201d\u6700\u5177\u4ee3\u8868\u6027\uff0c\u7136\u800c\u5176\u201c\u4e0e\u7cae\u4e89\u5730\u3001\u4e0e\u4eba\u4e89\u7cae\u201d\u7684\u539f\u6599\u4f9b\u5e94\u6a21\u5f0f\u5f15\u53d1\u4e86\u6781\u5927\u7684\u793e\u4f1a\u4e89\u8bae\uff1b\u4ee5\u6728\u8d28\u7ea4\u7ef4\u7d20\u7b49\u519c\u4e1a\u3001\u6797\u4e1a\u5e9f\u5f03\u7269\u4e3a\u539f\u6599\u7684\u7ea4\u7ef4\u7d20\u4e59\u9187\u5408\u6210\u6280\u672f\u7f13\u89e3\u4e86\u201c\u7cae\u98df\u4e59\u9187\u201d\u5728\u539f\u6599\u4f9b\u5e94\u4e0a\u7684\u4e0d\u8db3\uff0c\u4f46\u662f\u7ea4\u7ef4\u7d20\u539f\u6599\u7684\u9884\u5904\u7406\u53ca\u9176\u89e3\u7cd6\u5316\u8fc7\u7a0b\u9700\u8981\u6d88\u8017\u5927\u91cf\u80fd\u91cf\u3001\u6c34\u548c\u7ea4\u7ef4\u7d20\u9176\uff0c\u4ece\u800c\u6781\u5927\u5730\u62c9\u9ad8\u4e86\u751f\u4ea7\u6210\u672c\u3002\u4e0e\u751f\u7269\u70bc\u5236\u8fc7\u7a0b\u76f8\u6bd4\uff0c\u901a\u8fc7\u5149\u5408\u5fae\u751f\u7269\u5e73\u53f0\u5c06\u4e8c\u6c27\u5316\u78b3\u548c\u592a\u9633\u80fd\u76f4\u63a5\u8f6c\u5316\u4e3a\u4e59\u9187\u7684\u6280\u672f\u8def\u7ebf\uff08CO2 To Ethanol, CTE\uff09\u51cf\u5c11\u4e86\u539f\u6750\u6599\u9884\u5904\u7406\u3001\u5e95\u7269\u63d0\u70bc\u8fc7\u7a0b\u7684\u635f\u8017\uff0c\u4e5f\u8282\u7701\u4e86\u5bf9\u6de1\u6c34\u548c\u7528\u5730\u7684\u9700\u6c42\uff0c\u5728\u7ecf\u6d4e\u6027\u4e0e\u53ef\u6301\u7eed\u6027\u4e0a\u8868\u73b0\u51fa\u66f4\u5927\u7684\u6f5c\u529b\u4e0e\u4f18\u52bf\u3002<\/span><\/p>\n

\u4e2d\u56fd\u79d1\u5b66\u9662\u9752\u5c9b\u751f\u7269\u80fd\u6e90\u4e0e\u8fc7\u7a0b\u7814\u7a76\u6240\u5fae\u751f\u7269\u4ee3\u8c22\u5de5\u7a0b\u56e2\u961f\u5728\u84dd\u7ec6\u83cc\u5149\u5408\u751f\u7269\u5408\u6210\u4e59\u9187\u6280\u672f\u65b9\u9762\u53d6\u5f97\u4e86\u7cfb\u5217\u7814\u7a76\u8fdb\u5c55\u3002\u8be5\u56e2\u961f\u4ee5\u91cd\u8981\u7684\u6a21\u5f0f\u84dd\u7ec6\u83cc\u96c6\u80de\u85fbPCC6803\uff08Synechocystis sp. PCC6803\uff09\u4e3a\u5e95\u76d8\u85fb\u682a\uff0c\u5c06\u6765\u81ea\u8fd0\u52a8\u53d1\u9175\u5355\u80de\u83cc\u7684PdcZM-AdhIIZM\uff08\u4e19\u916e\u9178\u8131\u7fa7\u9176-II\u578b\u9187\u8131\u6c22\u9176\uff09\u9014\u5f84\u5bfc\u5165PCC6803\uff0c\u6253\u901a\u4e86\u4e59\u9187\u5149\u5408\u5408\u6210\u8def\u7ebf\uff0c\u5b9e\u73b0\u4e86\u5de5\u7a0b\u85fb\u682a\u4e2d\u4e59\u9187\u7684\u5408\u6210\u4e0e\u5206\u6ccc\uff1b\u9488\u5bf9\u84dd\u7ec6\u83cc\u751f\u7406\u548c\u4ee3\u8c22\u80cc\u666f\u7279\u6027\u5206\u6790\uff0c\u4f7f\u7528\u6765\u81ea\u96c6\u80de\u85fbPCC6803\u81ea\u8eab\u7684NADPH\u4f9d\u8d56\u578b\u7684II\u578b\u9187\u8131\u6c22\u9176\u57fa\u56e0slr1192\u66ff\u6362AdhIIZM\u540e\uff0c\u6210\u529f\u5730\u4f18\u5316\u4e86\u4e59\u9187\u5408\u6210\u9014\u5f84\u4e0e\u5e95\u76d8\u85fb\u682a\u7684\u9002\u914d\u6027\uff0c\u4f7f\u4e59\u9187\u4ea7\u91cf\u63d0\u9ad8\u4e8650%\uff1b\u5728\u6b64\u57fa\u7840\u4e0a\u7ed3\u5408\u7ade\u4e89\u6027\u9014\u5f84\u6572\u9664\u548c\u4ee3\u8c22\u9014\u5f84\u62f7\u8d1d\u6570\u5f3a\u5316\u7b49\u7b56\u7565\uff0c\u6700\u7ec8\u83b7\u5f97\u4e86\u5177\u6709\u8f83\u5f3a\u4e59\u9187\u5149\u5408\u5408\u6210\u80fd\u529b\u7684\u5de5\u7a0b\u85fb\u682aSyn-HZ24\uff0c\u5728\u67f1\u5f0f\u53cd\u5e94\u5668\u4e2d\u7ecf\u8fc728\u5929\u57f9\u517b\u540e\uff0c\u4e59\u9187\u4ea7\u91cf\u8fbe\u52305.5g\/L\uff0c\u5408\u6210\u901f\u73870.2g\/L\/day\uff0c\u5904\u4e8e\u56fd\u9645\u9886\u5148\u6c34\u5e73\u3002\u4e0a\u8ff0\u7ed3\u679c\u5960\u5b9a\u4e86\u8be5\u56e2\u961f\u5728\u84dd\u7ec6\u83cc\u4e59\u9187\u65b9\u5411\u4e0a\u7684\u7814\u7a76\u57fa\u7840\uff0c\u5df2\u7ecf\u53d1\u8868\u4e8e\u300a\u80fd\u6e90\u4e0e\u73af\u5883\u79d1\u5b66\u300b\uff08Energy & Environmental Science\uff0cGao, et al, 2012, 5:9857\u20139865\uff09\u3002<\/span><\/p>\n

\u4e3a\u4e86\u8fdb\u4e00\u6b65\u63d0\u5347\u84dd\u7ec6\u83cc\u5de5\u7a0b\u85fb\u682a\u4e59\u9187\u5149\u5408\u5408\u6210\u7684\u6f5c\u529b\uff0c\u8be5\u56e2\u961f\u91c7\u7528\u4f53\u5916\u91cd\u6784\u548c\u52a8\u6001\u5206\u6790\u7684\u7b56\u7565\u5bf9\u65b0\u53d1\u5c55\u7684PdcZM-slr1192\u9014\u5f84\u7684\u50ac\u5316\u7279\u6027\u8fdb\u884c\u4e86\u6df1\u5165\u6316\u6398\u3002\u9996\u5148\u8131\u79bb\u590d\u6742\u7684\u80de\u5185\u73af\u5883\u5728\u80de\u5916\u91cd\u65b0\u6784\u5efa\u8be5\u9014\u5f84\uff0c\u4ee5\u7eaf\u5316\u540e\u7684\u9176\uff08PdcZM\/slr1192\uff09\u3001\u5e95\u7269\uff08\u4e19\u916e\u9178\uff0cPyruvate\uff09\u3001\u8f85\u56e0\u5b50\uff08NADPH\/NADH\/TPP\uff09\u3001\u91d1\u5c5e\u79bb\u5b50\uff08Mg2+\uff09\u4ee5\u53ca\u4e2d\u95f4\u4ea7\u7269\uff08\u4e59\u919b\uff0cacetaldehyde\uff09\u5bf9\u6574\u4e2a\u9014\u5f84\u7684\u50ac\u5316\u6548\u7387\u5f71\u54cd\u5206\u522b\u8fdb\u884c\u5b9a\u91cf\u7684\u6ef4\u5b9a\u5206\u6790\uff0c\u7ed3\u679c\u53d1\u73b0\u5f71\u54cd\u4e59\u9187\u5408\u6210\u7684\u9650\u5236\u56e0\u7d20\u662fPdcZM\u800c\u975eslr1192\uff1b\u5728\u603b\u86cb\u767d\u6d53\u5ea6\u8bbe\u7f6e\u6052\u5b9a\u7684\u6761\u4ef6\u4e0b\uff0cPdcZM-slr1192\u7684\u6d53\u5ea6\u6bd4\u4e3a4:6\u65f6\uff0c\u5168\u9014\u5f84\u5177\u6709\u6700\u5927\u7684\u4e59\u9187\u5408\u6210\u50ac\u5316\u6d3b\u6027\u3002\u4e3a\u4e86\u9a8c\u8bc1Cell free\u6761\u4ef6\u4e0b\u83b7\u5f97\u7684\u7ed3\u679c\uff0c\u7814\u7a76\u4eba\u5458\u5728\u96c6\u80de\u85fbPCC6803\u4e2d\u6784\u5efa\u4e86PdcZM-slr1192\u6d53\u5ea6\u914d\u6bd4\u4e0d\u540c\u7684\u5de5\u7a0b\u83cc\u682a\uff0c\u901a\u8fc7\u4ee3\u8c22\u5de5\u7a0b\u7ed3\u5408\u9176\u6d3b\u4e0e\u86cb\u767d\u542b\u91cf\u5206\u6790\u5b9e\u9a8c\uff0c\u8bc1\u5b9e\u4e86\u73b0\u6709\u5de5\u7a0b\u85fb\u682a\u4e2dPdcZM\u7684\u8868\u8fbe\u91cf\u548c\u6d3b\u6027\u662f\u4e59\u9187\u5408\u6210\u80fd\u529b\u7684\u9996\u8981\u9650\u5236\u56e0\u7d20\u3002\u6b64\u5916\uff0c\u4f53\u5916\u91cd\u6784\u4f53\u7cfb\u4e2d\u7684\u6ef4\u5b9a\u6570\u636e\u7ed3\u5408\u84dd\u7ec6\u83cc\u80de\u5185\u4ee3\u8c22\u7269\u7684\u5b9e\u9645\u542b\u91cf\u5206\u6790\u8fd8\u663e\u793a\uff0c\u63d0\u9ad8NADPH\u548c\u4e19\u916e\u9178\u7684\u4f9b\u5e94\u91cf\u4e5f\u5e94\u8be5\u662f\u63d0\u9ad8\u4e59\u9187\u5408\u6210\u6548\u7387\u7684\u91cd\u8981\u9009\u62e9\u3002\u4e0a\u8ff0\u7ed3\u679c\u660e\u786e\u4e86\u84dd\u7ec6\u83cc\u4e59\u9187\u5149\u5408\u5de5\u7a0b\u85fb\u682a\u8fdb\u4e00\u6b65\u6539\u9020\u7684\u65b9\u5411\uff0c\u5df2\u7ecf\u53d1\u8868\u4e8e\u751f\u7269\u80fd\u6e90\u671f\u520aBiotechnology for Biofuels\uff08Luan, et al, 2015, 8:184\uff09\u3002<\/span><\/p>\n

\u57fa\u4e8e\u5df2\u7ecf\u5f00\u53d1\u7684\u84dd\u7ec6\u83cc\u4e59\u9187\u5149\u5408\u5de5\u7a0b\u85fb\u682a\uff0c\u8be5\u56e2\u961f\u53c8\u8fdb\u4e00\u6b65\u63a2\u7d22\u4e86\u5149\u5408\u7ec6\u80de\u5de5\u5382\u7684\u6269\u5927\u5316\u57f9\u517b\u6280\u672f\u3002\u5149\u5408\u751f\u7269\u5236\u9020\u901a\u5e38\u5728\u6237\u5916\u3001\u5f00\u653e\u5f0f\u3001\u672a\u706d\u83cc\u7684\u6761\u4ef6\u4e0b\u8fdb\u884c\uff0c\u56e0\u6b64\u7ecf\u5e38\u9762\u4e34\u5404\u79cd\u6a21\u5f0f\u7684\u751f\u7269\u6c61\u67d3\u7684\u4e25\u91cd\u5a01\u80c1\u8fdb\u800c\u5bfc\u81f4\u6269\u5927\u57f9\u517b\u7684\u5931\u8d25\u3002\u7814\u7a76\u4eba\u5458\u5728\u8fdb\u884c\u5de5\u7a0b\u85fb\u682aSyn-HZ24\u7684\u5f00\u653e\u5f0f\u3001\u89c4\u6a21\u5316\u57f9\u517b\u4e2d\u53d1\u73b0\uff0c\u5176\u4e59\u9187\u5408\u6210\u4e0e\u79ef\u7d2f\u8fc7\u7a0b\u53d7\u5230\u4e86\u5fae\u751f\u7269\u6c61\u67d3\u7684\u4e25\u91cd\u5f71\u54cd\u3002\u901a\u8fc7\u5206\u6790\u548c\u9274\u5b9a\uff0c\u786e\u5b9a\u4e86Pannonibacter phragmitetus\u4e3a\u4e59\u9187\u5149\u5408\u5408\u6210\u7684\u4e3b\u8981\u5a01\u80c1\u6765\u6e90\uff0c\u8be5\u83cc\u53ef\u4ee5\u4ee5\u4e59\u9187\u4e3a\u552f\u4e00\u78b3\u6e90\u8fdb\u884c\u751f\u957f\uff0c\u8fc5\u901f\u6d88\u8017\u5de5\u7a0b\u85fb\u682a\u5408\u6210\u7684\u4e59\u9187\u5e76\u5728\u57f9\u517b\u4f53\u7cfb\u4e2d\u5927\u91cf\u589e\u6b96\u3002\u901a\u8fc7\u5bf9Pannonibacter phragmitetus\u548cSyn-HZ24\u7684\u751f\u7406\u548c\u751f\u5316\u5206\u6790\uff0c\u7814\u7a76\u4eba\u5458\u63d0\u51fa\u63d0\u9ad8\u57f9\u517b\u4f53\u7cfbpH\u503c\u6765\u6291\u5236Pannonibacter phragmitetus\u4fb5\u67d3\u5e76\u6062\u590d\u4e59\u9187\u5149\u5408\u5408\u6210\u7684\u8bbe\u60f3\uff08Bicarbonate-based Integrated Carbon Capture System, BICCS\uff09\uff0c\u5e76\u5728\u5b9e\u9a8c\u5ba4\u67f1\u5f0f\u53cd\u5e94\u5668\u548c\u6237\u5916\u8584\u819c\u6302\u888b\u4e24\u79cd\u4f53\u7cfb\u4e0b\u8fdb\u884c\u4e86\u9a8c\u8bc1\uff0c\u7ed3\u679c\u8868\u660e\u8be5\u7b56\u7565\u53ef\u4ee5\u6709\u6548\u89e3\u51b3Syn-HZ24\u5728\u5f00\u653e\u5f0f\u57f9\u517b\u8fc7\u7a0b\u4e2d\u7684\u751f\u7269\u6c61\u67d3\u95ee\u9898\uff0c\u5728\u89c4\u6a21\u5316\u57f9\u517b9\u5929\u540e\u4e59\u9187\u4ea7\u91cf\u8fbe\u52300.9g\/L\uff0c\u800c\u5e38\u89c4pH\u6761\u4ef6\u4e0b\u7684\u5bf9\u7167\u4f53\u7cfb\u4e2d\u65e0\u4e59\u9187\u79ef\u7d2f\u3002\u6b64\u90e8\u5206\u5de5\u4f5c\u9274\u5b9a\u4e86\u4e00\u79cd\u5de5\u7a0b\u84dd\u7ec6\u83cc\u89c4\u6a21\u5316\u57f9\u517b\u8fc7\u7a0b\u4e2d\u65b0\u7684\u751f\u7269\u6c61\u67d3\u6a21\u5f0f\uff0c\u5e76\u9488\u5bf9\u6027\u5730\u8bbe\u8ba1\u3001\u9a8c\u8bc1\u4e86\u89e3\u51b3\u65b9\u6848\uff0c\u5bf9\u5149\u5408\u7ec6\u80de\u5de5\u5382\u57f9\u517b\u7684\u5de5\u7a0b\u5316\u3001\u89c4\u6a21\u5316\u53d1\u5c55\u6709\u666e\u904d\u7684\u501f\u9274\u610f\u4e49\u3002\u8be5\u5de5\u4f5c\u8fd1\u671f\u53d1\u8868\u4e8eBiotechnology for Biofuels\uff08Zhu, et al, 2017, 10:93\uff09\u3002<\/span><\/p>\n

\u4e0a\u8ff0\u7814\u7a76\u83b7\u5f97\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u3001\u201c863\u201d\u8ba1\u5212\u3001\u5c71\u4e1c\u7701\u6cf0\u5c71\u5b66\u8005\u9879\u76ee\u3001\u5c71\u4e1c\u7701\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u4ee5\u53ca\u9752\u5c9b\u5e02\u521b\u65b0\u9886\u519b\u4eba\u624d\u9879\u76ee\u7684\u652f\u6301\u3002<\/span><\/p>\n

\"\u56fe1.<\/a>
<\/span> \u56fe1. \u91c7\u7528BICCS\u8865\u78b3\u7b56\u7565\u63d0\u9ad8\u57f9\u517b\u4f53\u7cfbpH\u503c\u4ee5\u6291\u5236\u6742\u83cc\u6c61\u67d3\u5e76\u6062\u590d\u4e59\u9187\u5408\u6210<\/span><\/figcaption><\/figure>\n
\"\u56fe2.<\/a>
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