{"id":7494,"date":"2020-07-02T10:35:42","date_gmt":"2020-07-02T02:35:42","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=7494"},"modified":"2020-07-02T10:36:26","modified_gmt":"2020-07-02T02:36:26","slug":"led-light-regulation-and-nitrogen-supplement-to-phaeodactylum-tricornutum","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/led-light-regulation-and-nitrogen-supplement-to-phaeodactylum-tricornutum.html","title":{"rendered":"LED\u8c03\u5149\u548c\u8865\u6c2e\u8054\u5408\u7b56\u7565\u63d0\u9ad8\u517c\u517b\u4e09\u89d2\u8910\u6307\u85fb\u7684\u5ca9\u85fb\u9ec4\u7d20\u4ea7\u91cf"},"content":{"rendered":"

\u8f6c\u81ea\uff1a\u5fae\u85fb\u6280\u672f\u4e0e\u4ea7\u4e1a<\/span><\/p>\n

\u534e\u5357\u7406\u5de5\u5927\u5b66\u5728\u6d77\u6d0b\u5fae\u85fb\u751f\u4ea7\u5ca9\u85fb\u9ec4\u7d20\u7814\u7a76\u65b9\u9762\u53d6\u5f97\u91cd\u8981\u7a81\u7834\u2014\u2014LED\u8c03\u5149\u548c\u8865\u6c2e\u8054\u5408\u7b56\u7565\u63d0\u9ad8\u517c\u517b\u4e09\u89d2\u8910\u6307\u85fb\u7684\u5ca9\u85fb\u9ec4\u7d20\u4ea7\u91cf<\/span><\/p>\n

\u5ca9\u85fb\u9ec4\u7d20\uff08Fucoxanthin\uff09\u662f\u4e00\u79cd\u5e7f\u6cdb\u5b58\u5728\u4e8e\u6d77\u6d0b\u85fb\u7c7b\u7684\u7c7b\u80e1\u841d\u535c\u7d20\uff0c\u5360\u6574\u4e2a\u751f\u7269\u5708\u4e2d\u6240\u6709\u7c7b\u80e1\u841d\u535c\u7d20\u542b\u91cf\u768410%\u4ee5\u4e0a\u3002\u5ca9\u85fb\u9ec4\u7d20\u5177\u6709\u6297\u764c\u3001\u6297\u80a5\u80d6\u3001\u9884\u9632\u7cd6\u5c3f\u75c5\u53ca\u963f\u5c14\u5179\u6d77\u9ed8\u75c7\u7b49\u529f\u6548\uff0c\u5728\u751f\u7269\u533b\u836f\u548c\u529f\u80fd\u98df\u54c1\u9886\u57df\u5177\u6709\u91cd\u8981\u5e94\u7528\u4ef7\u503c\u3002\u76ee\u524d\u5546\u4e1a\u5316\u5ca9\u85fb\u9ec4\u7d20\u7684\u4e3b\u8981\u6765\u6e90\u662f\u6d77\u5e26\u3001\u88d9\u5e26\u83dc\u7b49\u5927\u578b\u8910\u85fb\uff0c\u8fd9\u7c7b\u539f\u6599\u4e2d\u5ca9\u85fb\u9ec4\u7d20\u542b\u91cf\u6781\u4f4e\uff08\u4ec5\u7ea6\u4e3a\u5e72\u91cd\u76840.01%\uff09\uff0c\u63d0\u53d6\u56f0\u96be\u3001\u52a0\u5de5\u6210\u672c\u9ad8\u4e14\u4ea7\u7269\u7eaf\u5ea6\u4f4e\uff0c\u96be\u4ee5\u6ee1\u8db3\u65e5\u76ca\u589e\u957f\u7684\u5e02\u573a\u9700\u6c42\u3002\u6d77\u6d0b\u7845\u85fb\u4e09\u89d2\u8910\u6307\u85fb\uff08<\/strong>Phaeodactylum tricornutum<\/em><\/strong>\uff09\u751f\u957f\u901f\u5ea6\u5feb\uff0c\u53ef\u8fdb\u884c\u81ea\u517b\u548c\u517c\u517b\u57f9\u517b\uff0c\u81ea\u517b\u4e0b\u5176\u5ca9\u85fb\u9ec4\u7d20\u542b\u91cf\u9ad8\u8fbe\u5e72\u91cd\u7684<\/strong>1%~6%<\/strong>\uff0c\u662f\u5927\u578b\u6d77\u85fb\u7684\u4e0a\u767e\u500d<\/strong>\u3002<\/strong>\u4e09\u89d2\u8910\u6307\u85fb\u6765\u6e90\u7684\u5ca9\u85fb\u9ec4\u7d20\u85fb\u6cb9\u5df2\u83b7\u5f97\u7f8e\u56fdFDA\u8ba4\u8bc1\uff0c\u4f5c\u4e3a\u809d\u810f\u8102\u80aa\u63a7\u5236\u7684\u81b3\u98df\u8865\u5145\u5242\uff0c\u662f\u56fd\u9645\u5e02\u573a\u7684\u65b0\u5ba0\u3002<\/span><\/p>\n

\u76ee\u524d\uff0c\u4e09\u89d2\u8910\u6307\u85fb\u7684\u57f9\u517b\u4e3b\u8981\u5728\u81ea\u517b\u6761\u4ef6\u4e0b\u8fdb\u884c\uff0c\u751f\u7269\u91cf\u6d53\u5ea6\u4f4e\u3001\u57f9\u517b\u65f6\u95f4\u957f\uff0c\u4e0d\u5229\u4e8e\u9ad8\u6548\u751f\u4ea7\u5ca9\u85fb\u9ec4\u7d20\u3002\u524d\u4eba\u7814\u7a76\u8868\u660e\uff0c\u4e09\u89d2\u8910\u6307\u85fb\u5728\u517c\u517b\u6761\u4ef6\u4e0b\u751f\u7269\u91cf\u5e72\u91cd\u53ef\u8fbe3~15 g\/L\uff0c\u4f46\u7c7b\u80e1\u841d\u535c\u7d20\u542b\u91cf\u901a\u5e38\u53ea\u6709\u5e72\u91cd\u76840.5%~0.7%\u3002\u5f00\u53d1\u4e09\u89d2\u8910\u6307\u85fb\u5728\u517c\u517b\u6761\u4ef6\u4e0b\u5feb\u901f\u751f\u957f\u53ca\u9ad8\u6548\u79ef\u7d2f\u5ca9\u85fb\u9ec4\u7d20\u7684\u5173\u952e\u6280\u672f\u662f\u76ee\u524d\u4e9f\u5f85\u7a81\u7834\u7684\u74f6\u9888\u3002<\/span><\/section>\n

\u534e\u5357\u7406\u5de5\u5927\u5b66\u9b4f\u4e1c\u6559\u6388\u8bfe\u9898\u7ec4\u7cfb\u7edf\u7814\u7a76\u4e86\u4e09\u89d2\u8910\u6307\u85fb\u54cd\u5e94\u4e0d\u540c\u5149\u8d28\u4e0e\u8865\u6c2e\u7b56\u7565\u7684\u7279\u6027\uff0c\u5efa\u7acb\u4e86\u4e24\u9636\u6bb5\u517c\u517b\u57f9\u517b\u6280\u672f\uff0c\u5b9e\u73b0\u4e86\u5ca9\u85fb\u9ec4\u7d20\u5728\u8be5\u85fb\u4e2d\u7684\u9ad8\u6548\u79ef\u7d2f\u3002\u7814\u7a76\u8bba\u6587\u201cImproving fucoxanthin production in mixotrophic culture of marine diatom Phaeodactylum tricornutum b<\/em>y LED light shift and nitrogen supplementation<\/strong>\u201d\u8fd1\u671f\u53d1\u8868\u5728\u56fd\u9645\u751f\u7269\u5de5\u7a0b\u671f\u520aFrontiers in Bioengineering and Biotechnology<\/strong>\u3002\u00a0<\/span><\/p>\n

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\u8be5\u7814\u7a76\u5148\u5728\u6447\u74f6\u4e2d\u4f18\u5316\u4e86\u4e09\u89d2\u8910\u6307\u85fb\u7684\u517c\u517b\u57f9\u517b\u6761\u4ef6\uff0c\u53d1\u73b0\u5728\u8f83\u9ad8\u63a5\u79cd\u5bc6\u5ea6\uff081\u00d7107<\/sup>cells\/mL\uff09\u548c\u8f83\u4f4e\u5149\u7167\u5f3a\u5ea6\uff0820 \u03bcmol\/m2<\/sup>\/s\uff09\u4e0b\uff0c\u6709\u5229\u4e8e\u4e09\u89d2\u8910\u6307\u85fb\u751f\u7269\u91cf\u548c\u5ca9\u85fb\u9ec4\u7d20\u79ef\u7d2f\u3002\u5728\u7ea2\u84dd\u6df7\u5408\u5149\uff086:1\uff09\u548c\u7eaf\u84dd\u5149\u7167\u5c04\u4e0b\uff0c\u53ef\u5206\u522b\u8fbe\u5230\u6700\u5927\u751f\u7269\u91cf\u6d53\u5ea6\uff085.53 g\/L\uff09\u548c\u6700\u9ad8\u5ca9\u85fb\u9ec4\u7d20\u542b\u91cf\uff0816.28 mg\/g\uff09\u3002\u56e0\u6b64\uff0c\u5728\u7b2c\u4e00\u9636\u6bb5\u91c7\u7528\u7ea2\u84dd\u6df7\u5408\u5149\uff086:1\uff09\u7528\u4e8e\u4fc3\u8fdb\u7ec6\u80de\u751f\u957f\uff0c\u5728\u7b2c\u4e8c\u9636\u6bb5\u91c7\u7528\u52a0\u5f3a\u84dd\u5149\u53ca\u8865\u6c2e\u64cd\u4f5c\uff0c\u7528\u4e8e\u4fc3\u8fdb\u751f\u7269\u91cf\u53ca\u5ca9\u85fb\u9ec4\u7d20\u79ef\u7d2f\u3002\u901a\u8fc7\u8f6c\u6362\u5149\u8d28\u548c\u8865\u6c2e\u7684\u4e24\u6b65\u6cd5\u64cd\u4f5c\uff0c<\/strong>\u4e09\u89d2\u8910\u6307\u85fb\u5728\u7b2c\u4e8c\u9636\u6bb5\u83b7\u5f97\u4e86\u6700\u9ad8\u751f\u7269\u91cf\u6d53\u5ea6\uff08<\/strong>6.52 g\/L<\/strong>\uff09\u548c\u5ca9\u85fb\u9ec4\u7d20\u4ea7\u7387\uff08<\/strong>8.22mg\/L\/d<\/strong>\uff09<\/strong>\u3002<\/strong>\u4e0e\u7b2c\u4e00\u9636\u6bb5\u76f8\u6bd4\uff0c\u6bcf\u4e2a\u7ec6\u80de\u5185\u5ca9\u85fb\u9ec4\u7d20\u542b\u91cf\uff08pg\/cell\uff09\u63d0\u5347\u4e8612%\u3002\u5bf9\u5ca9\u85fb\u9ec4\u7d20\u5408\u6210\u9014\u5f84\u4e2d\u5173\u952e\u57fa\u56e0\u7684\u8f6c\u5f55\u6c34\u5e73\u7684qRT-PCR\u5206\u6790\u8868\u660e\uff0c\u5728\u7b2c\u4e8c\u9636\u6bb5\u4e2d\u7f16\u7801\u516b\u6c22\u756a\u8304\u7ea2\u7d20\u5408\u6210\u9176\uff08<\/strong>PSY<\/strong>\uff09\u3001\u7389\u7c73\u9ec4\u8d28\u73af\u6c27\u5316\u9176\uff08<\/strong>ZEP<\/strong>\uff09\u548c\u5ca9\u85fb\u9ec4\u7d20<\/strong>–<\/strong>\u53f6\u7eff\u7d20\u590d\u5408\u86cb\u767d\u4f53<\/strong>b <\/strong>\uff08<\/strong>FCPb<\/strong>\uff09\u7684\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u663e\u8457\u4e0a\u8c03\uff0c\u800c\u7f16\u7801\u7d2b\u9ec4\u7d20\u73af\u6c27\u5316\u9176\uff08<\/strong>VDE<\/strong>\uff09\u7684\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u663e\u8457\u4e0b\u8c03<\/strong>\uff0c\u7ed3\u679c\u663e\u8457\u63d0\u5347\u4e86\u5ca9\u85fb\u9ec4\u7d20\u542b\u91cf\u3002<\/span><\/section>\n
\n
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\u56fe2 <\/span>\u4e24\u6b65\u6cd5\u517c\u517b\u57f9\u517b\u4e09\u89d2\u8910\u6307\u85fb\u7684\u751f\u4ea7\u6027\u80fd\u4e0e\u5ca9\u85fb\u9ec4\u7d20\u5408\u6210\u9014\u5f84\u4e2d\u5173\u952e\u57fa\u56e0\u8f6c\u5f55\u6c34\u5e73\u8c03\u63a7<\/span><\/figcaption><\/figure>\n<\/section>\n
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    \n
  1. \n
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  2. \n
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  3. \n
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  4. \n
    LED\u8c03\u5149\u548c\u8865\u6c2e\u8054\u5408\u7b56\u7565\u53ef\u4ee5\u83b7\u5f97\u6700\u9ad8\u5ca9\u85fb\u9ec4\u7d20\u4ea7\u73878.22 mg\/L\/d\uff1b<\/span><\/section>\n<\/li>\n
  5. \n
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    \u3010\u8bba\u6587\u51fa\u5904\u3011<\/strong><\/span><\/p>\n

    RunqingYang, Dong Wei. Improving fucoxanthin production in mixotrophic culture of marine diatom Phaeodactylum tricornutum <\/em>by LED light shift and nitrogen supplementation. Frontiers in Bioengineering and Biotechnology. 2020. doi: 10.3389\/fbioe.2020.00820.<\/span><\/section>\n

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    \u3010\u9b4f\u4e1c\u6559\u6388\u7b80\u4ecb\u3011<\/strong><\/span><\/p>\n

    \u534e\u5357\u7406\u5de5\u5927\u5b66\u98df\u54c1\u79d1\u5b66\u4e0e\u5de5\u7a0b\u5b66\u9662\u6559\u6388\u3001\u535a\u58eb\u751f\u5bfc\u5e08\u3002\u7814\u7a76\u65b9\u5411\u4e3a\u751f\u7269\u8f6c\u5316\u548c\u8425\u517b\u5de5\u7a0b\u2014\u2014\u9762\u5411\u529f\u80fd\u98df\u54c1\u3001\u73af\u5883\u4fee\u590d\u548c\u5e9f\u5f03\u7269\u8d44\u6e90\u5316\u5229\u7528\u7684\u5de5\u4e1a\u751f\u7269\u6280\u672f\uff0c\u4e3b\u8981\u5305\u62ec\uff1a<\/span><\/section>\n

    1\u3001\u56f4\u7ed5\u5de5\u4e1a\u751f\u7269\u6280\u672f\u7684\u6838\u5fc3\u5185\u5bb9\uff0c\u9488\u5bf9\u5bcc\u542b\u751f\u7269\u6d3b\u6027\u7269\u8d28\u548c\u9ad8\u6548\u73af\u5883\u4fee\u590d\u7684\u5fae\u85fb\uff08\u9175\u6bcd\uff09\u4f18\u826f\u79cd\u8d28\uff0c\u5efa\u7acb\u201c\u7ec4\u5b66\u201d\uff08Omics\uff09\u6280\u672f\u5e73\u53f0\uff0c\u5728\u8f6c\u5f55\u7ec4\u3001\u4ee3\u8c22\u7ec4\uff08\u8102\u7ec4\uff09\u3001\u7ec6\u80de\u7ec4\u3001\u85fb\u9645\u5fae\u751f\u7269\u7ec4\u6c34\u5e73\u4e0a\uff0c\u91cd\u70b9\u63a2\u7d22\u8425\u517b\u4e0e\u73af\u5883\u56e0\u5b50\u5bf9\u5fae\u85fb\u4e0e\u85fb\u9645\u5fae\u751f\u7269\u7684\u4f5c\u7528\u673a\u5236\u3001\u5fae\u85fb\u5149\u5408\u4f5c\u7528\u548c\u5149\u53d1\u9175\u8fc7\u7a0b\u4e2d\u78b3\u6c2e\u78f7\u9ad8\u6548\u751f\u7269\u8f6c\u5316\u53ca\u4ee3\u8c22\u8c03\u63a7\u673a\u5236\u7684\u57fa\u7840\u79d1\u5b66\u95ee\u9898\uff1b<\/span><\/p>\n

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