{"id":8510,"date":"2022-06-02T11:02:39","date_gmt":"2022-06-02T03:02:39","guid":{"rendered":"https:\/\/www.leadingtec.cn\/?p=8510"},"modified":"2022-06-02T11:02:42","modified_gmt":"2022-06-02T03:02:42","slug":"%e5%be%b7%e5%9b%bd%e5%9f%ba%e5%b0%94%e5%a4%a7%e5%ad%a6%e6%8f%ad%e7%a4%ba%e8%93%9d%e8%97%bb%e5%8e%8c%e6%b0%a7%e9%85%b6nife%e6%b0%a2%e5%8c%96%e9%85%b6%e5%9c%a8%e6%9c%89%e6%b0%a7%e6%9d%a1%e4%bb%b6","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/%e5%be%b7%e5%9b%bd%e5%9f%ba%e5%b0%94%e5%a4%a7%e5%ad%a6%e6%8f%ad%e7%a4%ba%e8%93%9d%e8%97%bb%e5%8e%8c%e6%b0%a7%e9%85%b6nife%e6%b0%a2%e5%8c%96%e9%85%b6%e5%9c%a8%e6%9c%89%e6%b0%a7%e6%9d%a1%e4%bb%b6.html","title":{"rendered":"\u5fb7\u56fd\u57fa\u5c14\u5927\u5b66\u63ed\u793a\u84dd\u85fb\u538c\u6c27\u9176NiFe\u6c22\u5316\u9176\u5728\u6709\u6c27\u6761\u4ef6\u4e0b\u7684\u91cd\u8981\u529f\u80fd\u673a\u5236"},"content":{"rendered":"
5\u670831\u65e5\uff0c\u5fb7\u56fd\u57fa\u5c14\u5927\u5b66\u690d\u7269\u6240Kirstin Gutekunst\u56e2\u961f\u4e8eFrontiers in Microbiology\u4e0a\u53d1\u8868\u201cSynechocystis sp. PCC 6803 requires the bidirectional hydrogenase to metabolize glucose and arginine under oxic conditions\u201d\u8bba\u6587\u3002\u8be5\u8bba\u6587\u53d1\u73b0\u84dd\u85fb\u9700\u8981\u4f9d\u9760\u53cc\u5411NiFe\u6c22\u5316\u9176\u6765\u7ef4\u6301\u5b58\u5728\u6709\u673a\u78b3\u6e90\u548c\u6c2e\u6e90\u7684\u6761\u4ef6\u4e0b\u7684\u6709\u6c27\u751f\u957f\u3002\u8fd9\u4e2a\u53d1\u73b0\u662f\u4ee4\u4eba\u610f\u5916\u7684\uff0c\u56e0\u4e3a\u8fc7\u53bb\u4eba\u4eec\u90fd\u8ba4\u4e3a\u84dd\u85fb\u7684\u8fd9\u4e2aNiFe\u6c22\u5316\u9176\u662f\u538c\u6c27\u7684\u3002\u8fd9\u7bc7\u8bba\u6587\u663e\u793a\u8be5\u6761\u4ef6\u4e0b\u5e76\u6ca1\u6709\u6c22\u6c14\u7684\u4ea7\u751f\uff0c\u610f\u5473\u7740\u8fd9\u4e2a\u9176\u5728\u6709\u6c27\u6761\u4ef6\u4e0b\u5e94\u8be5\u5b58\u5728\u7740\u4e00\u79cd\u81f3\u4eca\u672a\u77e5\u7684\u529f\u80fd\u3002\u5b83\u53ef\u80fd\u77ed\u671f\u4f5c\u4e3a\u4e00\u79cd\u7535\u5b50\u9600\u6765\u5feb\u901f\u54cd\u5e94\u8461\u8404\u7cd6\u548c\u7cbe\u6c28\u9178\u7684\u4f9b\u5e94\uff0c\u4e5f\u53ef\u80fd\u957f\u671f\u62c5\u8d1f\u7740\u5c06\u8461\u8404\u7cd6\u548c\u7cbe\u6c28\u9178\u6c27\u5316\u4ea7\u751f\u7684\u7535\u5b50\u4f20\u8f93\u5230\u5149\u5408\u7535\u5b50\u4f20\u9012\u94fe\u4e2d\u7684\u529f\u80fd\u3002\u6216\u8005\u8fd9\u4e2a\u6c22\u5316\u9176\u4e5f\u53ef\u80fd\u901a\u8fc7NADP(H)\u3001NAD(H)\u548cferredoxin\u5e93\u4e4b\u95f4\u7684\u7535\u5b50\u7a7f\u68ad\u4ee5\u6b64\u6765\u5e73\u8861\u7ec6\u80de\u4e2d\u7684\u6c27\u5316\u8fd8\u539f\u538b\u529b\u3002<\/p>
\u84dd\u85fb\u6a21\u5f0f\u7269\u79cdSynechocystis sp. PCC 6803\u542b\u6709\u4e00\u4e2a\u53cc\u5411NiFe\u6c22\u5316\u9176\uff0cHoxEFUYH\u3002\u5b83\u65e2\u53ef\u4ee5\u50ac\u5316\u5728\u9ed1\u6697\u7684\u6761\u4ef6\u4e2d\u4ea7\u751f\u6c22\u6c14\uff08\u53d1\u9175\u4ea7\u6c22\u6a21\u5f0f\uff09\uff0c\u4e5f\u53ef\u4ee5\u50ac\u5316\u7ec6\u80de\u7531\u9ed1\u6697\u5411\u5149\u7167\u8f6c\u6362\u65f6\u4ea7\u751f\u6c22\u6c14\uff08\u5149\u5408\u4ea7\u6c22\u6a21\u5f0f\uff09\uff08Gutekunst et al. 2014\uff09\u3002\u540e\u8005\u6301\u7eed\u65f6\u95f4\u8f83\u77ed\uff0c\u968f\u540e\u5149\u5408\u4f5c\u7528\u79ef\u7d2f\u4ea7\u751f\u7684\u6c22\u6c14\u4f1a\u88abHoxEFUYH\u6c27\u5316\u5438\u6536\uff0c\u4ea7\u751f\u7684\u7535\u5b50\u5f88\u5927\u53ef\u80fd\u4f1a\u7ecf\u8d28\u4f53\u918cPQ\u4f20\u9012\u5230\u5149\u5408\u7535\u5b50\u4f20\u9012\u94fe\u3002\u4e00\u65e6\u5149\u5408\u4f5c\u7528\u4ea7\u751f\u7684\u6c27\u6c14\u5bcc\u96c6\uff0c\u7ec6\u80de\u5c31\u65e2\u4e0d\u80fd\u4ea7\u751f\u6c22\u6c14\u4e5f\u4e0d\u80fd\u6c27\u5316\u5438\u6536\u6c22\u6c14\u4e86\u3002\u76ee\u524d\u7684\u7814\u7a76\u6a21\u578b\u8ba4\u4e3a\u6c27\u6c14\u4f1a\u5728NiFe\u6c22\u5316\u9176\u7684\u6d3b\u6027\u4f4d\u70b9\u901a\u8fc7\u5f62\u6210\u4e00\u4e2a\u7f9f\u57fa\uff08OH-\uff09\u8fde\u63a5Ni\u79bb\u5b50\u548cFe\u79bb\u5b50\u8fdb\u800c\u963b\u65ad\u6c22\u6c14\u7684\u5faa\u73af (Caserta et al.2020)\u3002\u7531\u4e8e\u5b83\u7684\u6613\u4e8e\u5931\u6d3b\uff0c\u6c22\u5316\u9176\u88ab\u8ba4\u4e3a\u662f\u538c\u6c27\u7684\u3002\u9700\u8981\u6ce8\u610f\u7684\u662f\uff0c\u8fd9\u79cd\u6c22\u5316\u9176\u5e7f\u6cdb\u5b58\u5728\u4e8e\u84dd\u85fb\u4e2d\uff08Barz M, et al. 2010\uff09\uff0c\u800c\u81ea\u7136\u754c\u7684\u84dd\u85fb\u4e3b\u8981\u751f\u6d3b\u5728\u6709\u6c27\u7684\u6761\u4ef6\u4e2d\u3002\u6b64\u5916\u4eba\u4eec\u8fd8\u53d1\u73b0\u6709\u6c27\u6761\u4ef6\u4e0b\uff0c\u6c22\u5316\u9176\u5728\u84dd\u85fbSynechocystis\u4e2d\u4f1a\u6301\u7eed\u8868\u8fbe\uff0c\u4f53\u5185\u5b9e\u9a8c\u4e5f\u53ef\u4ee5\u68c0\u6d4b\u5230\u9664\u5b8c\u6574\u6c22\u5316\u9176\u4e94\u805a\u4f53HoxEFUYH\u4e4b\u5916\u7684\u5176\u4ed6\u5b50\u590d\u5408\u7269\u3002\u7136\u800c\uff0c\u81f3\u4eca\u672a\u77e5\u8fd9\u4e9b\u5b50\u590d\u5408\u4f53\u662f\u5426\u5c65\u884c\u67d0\u4e9b\u529f\u80fd\u3002<\/p>
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