CSI medium
?# |
組份 |
用量 |
母液濃度 |
1 |
Ca(NO3) ·4H2O |
1 mL/L |
15 g/100 mL dH2O |
2 |
KNO3 |
1 mL/L |
10g/100 mL dH2O |
3 |
MgSO4·7H2O |
?1 mL/L |
4g/100 mL dH2O |
4 |
β-Na2 glycerophosphate·5H2O |
?1 mL/L |
2 .5g/100 mL dH2O |
5 |
Vitamin B12 |
0.1ug/L |
|
6 |
Biotin |
0.1ug/L |
|
7 |
Thiamine HCl |
10ug/L |
|
8 |
PIV* |
6ml/L |
|
9 |
HEPES |
?0.5g/L |
|
10 |
Na2SiO3·9H2O |
0.1g/L |
|
9 |
土壤提取液** |
?30ml/L |
PH調(diào)到7.0
*PIV
組份 |
濃度 |
Na2EDTA |
0.75g/L dH2O |
MnCl2·4H2O |
0.041g/L dH2O |
ZnCl2·7H2O |
0.005g/L dH2O |
Na2MoO4·2H2O |
0.004g/L dH2O |
FeCl3·6 H2O |
0.097g/L dH2O |
CoCl2.6 H2O |
0.002g/L dH2O |
**土壤提取液
土壤提取液配制方法: 取花園土未施過肥200g置于燒杯或三角瓶中,加入蒸餾水1000毫升,瓶口用透氣塞封口,在水浴中沸水加熱3小時,冷卻,沉淀24小時,此過程連續(xù)進行 3次,然后過濾,取上清液,于高壓滅菌鍋中滅菌后于4℃冰箱中保存?zhèn)溆谩?/div>