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一株產(chǎn)油微藻——小球藻的純化鑒定與培養(yǎng)基的篩選
Identification of an Oil-producing Microalgae——Chlorella and Its Medium Selected
作者：梁穎[1,2] 馮俊麗[1] 徐方嬌[1,2] 陳集雙[2,3]
Liang Ying,Feng Junli,Xu Fangjiao,Chen Jishuang,（1.Intitute of Biotechnology,Zhejiang Sci-Tech University,Hangzhou,310018,China; 2.Marine Industrial Institute,Nanjing University of Technology,Dafeng,224145,China; 3.College of Life Science and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing,210009,China）
機(jī)構(gòu)地區(qū)：[1]浙江理工大學(xué)生物工程研究所,杭州310018 [2]南京工業(yè)大學(xué)大豐海洋產(chǎn)業(yè)研究院,江蘇大豐224145 [3]南京工業(yè)大學(xué)生物與制藥工程學(xué)院,南京210009
出處：《科技通報(bào)》 2013年第29卷第3期 40-46頁(yè),共7頁(yè)
Bulletin of Science and Technology
基金：國(guó)家自然基金資助（No.30971898）
摘要：從南京工業(yè)大學(xué)生物與制藥工程學(xué)院獲得一株小球藻藻種,經(jīng)劃線培養(yǎng)數(shù)代后,得到分離純化的小球藻,18SrDNA鑒定確定其種屬為普通小球藻。小球藻的常見(jiàn)幾種培養(yǎng)基篩選結(jié)果表明,BG11培養(yǎng)基最適合該小球藻的生長(zhǎng)。用牛糞浸出液代替?zhèn)鹘y(tǒng)培養(yǎng)基培養(yǎng)小球藻的梯度對(duì)照實(shí)驗(yàn)中,發(fā)現(xiàn)牛糞浸出液培養(yǎng)小球藻的效果很好,且濃度越高,生長(zhǎng)狀況越好。同時(shí)還對(duì)小球藻的粗脂含量做了測(cè)定,每克小球藻干粉中粗脂的含量約為0.206 g,占小球藻干重的20%左右。本研究為新能源的開(kāi)發(fā)和利用積累了信息。
In this study the Chlorella species came from Biotechnology and Pharmaceutical Engineering of Nanjing University of technology.It was purified by crossed culture after several generations,and was determined to be Chlorella vulgaris strain by 18S rDNA sequence.The medium selected result shows that BG11 medium was the best for the growth of Chlorella.Then, the cow dung leaching solution was used for Chlorella culture insteaded of the traditional medium.The results showed that Chlorella growth well in cow dung leaching solution,and the higher of the concentration,the better for Chlorella growth.At the same time,we measured the Chlorella crude fat content of the cultived Chlorella and the result was that about 0.206g crude fat in each grams of Chlorella powder,which accounted for about 20% of Chlorella dry weight.These results have an important significance for the development of new energy.
關(guān) 鍵 詞：產(chǎn)油微藻 小球藻 牛糞浸出液 BG11培養(yǎng)基 粗脂
oil-producing microalgae Chlorella cow dung leaching solution BG11 medium crude fat
分 類 號(hào)：Q949.9 [生物科學(xué) > 植物學(xué) > 植物分類學(xué)(系統(tǒng)植物學(xué))]