Walnes 培養(yǎng)基 (Walnes medium)是一種國外常用的海水藻類培養(yǎng)基，光語生物提供商品裝培養(yǎng)基，客戶也可以根據(jù)如下配方配置。
Walne’s Medium Composition
From https://biocyclopedia.com/index/algae/algal_culturing/walnes_medium_composition.php
| Reagents | Per Liter Seawater |
|---|---|
| Solution A | 1.0 ml |
| Solution C | 0.1 ml |
| Solution D (to add for diatoms) | 2.0 ml |
| Solution A | |
| FeCl3?* 6H2O | 1.3 g |
| MnCl2?* 4H2O | 0.4 g |
| H3BO3 | 33.6 g |
| Na2EDTA | 45.0 g |
| NaH2PO4?* 2H2O | 20.0 g |
| NaNO3 | 100.0 g |
| Solution B | 1 ml |
| Solution B per 100ml | |
| ZnCl2 | 2.1 g |
| CoCl2?* 6H2O | 2.0 g |
| (NH4)6Mo7O24?* 4H2O | 0.9 g |
| CuSO4?* 5H2O | 2.0 g |
| Concentrated HCl | 10 ml |
| Solution C per 200ml | |
| Thiamine HCl (Vitamin B1) | 0.2 g |
| Cyanocobalamin (Vitamin B12) | 10 mg |
| Solution D (per liter) | |
| Na2SiO3?* 5H2O | 40.0 g |
